Dutkiewicz Grazyna, Binczak-Kuleta Agnieszka, Pawlik Andrzej, Safranow Krzysztof, Wisniewska Magda, Ciechanowicz Andrzej, Dziedziejko Violetta, Ciechanowski Kazimierz, Domanski Leszek
Department of Nephrology, Transplantology and Internal Medicine of Pomeranian Medical University, Szczecin, Poland.
Ann Transplant. 2010 Jul-Sep;15(3):30-4.
The glutathione peroxidase/glutathione system is a major defense in oxidative stress. Glutathione peroxidase (GPx) is a selenium-containing antioxidant enzyme that effectively reduces hydrogen peroxide and lipid peroxides to water and lipid alcohols, respectively, and in turn oxidizes glutathione to glutathione disulfide. Previous studies have shown that the activity of glutathione peroxidase is genetically determined and is associated with polymorphisms in GPX1 gene. The aim of the present study was to examine the association between the C599T polymorphism in the glutathione peroxidase (GPX1) gene and delayed graft function of kidney allografts, acute rejection and chronic allograft nephropathy.
MATERIAL/METHODS: One hundred eighty-seven recipients of first cadaveric renal transplants from the Department of Nephrology, Transplantology and Internal Medicine of Pomeranian Medical University were included in this retrospective study. Genotyping of C599T polymorphism in the GPX1 gene was performed using PCR-RFLP method.
There were no significant associations between this polymorphism and delayed graft function, acute rejection and chronic allograft nephropathy.
The present results suggest that GPX1 C599T polymorphism has no influence on the graft function in the first phase after transplantation, as well as on the acute kidney graft rejection and chronic allograft nephropathy.
谷胱甘肽过氧化物酶/谷胱甘肽系统是氧化应激中的主要防御机制。谷胱甘肽过氧化物酶（GPx）是一种含硒的抗氧化酶，能分别有效地将过氧化氢和脂质过氧化物还原为水和脂质醇，进而将谷胱甘肽氧化为谷胱甘肽二硫化物。先前的研究表明，谷胱甘肽过氧化物酶的活性由基因决定，并与GPX1基因的多态性相关。本研究的目的是探讨谷胱甘肽过氧化物酶（GPX1）基因C599T多态性与肾移植受者移植肾功能延迟恢复、急性排斥反应及慢性移植肾肾病之间的关系。
材料/方法：本回顾性研究纳入了187例来自波美拉尼亚医科大学肾脏病学、移植学和内科的首次尸体肾移植受者。采用PCR-RFLP方法对GPX1基因C599T多态性进行基因分型。
该多态性与移植肾功能延迟恢复、急性排斥反应及慢性移植肾肾病之间无显著相关性。
目前的结果表明，GPX1基因C599T多态性对移植后第一阶段的移植肾功能以及急性肾移植排斥反应和慢性移植肾肾病均无影响。
