Kłoda Karolina, Domanski Leszek, Pawlik Andrzej, Kurzawski Mateusz, Safranow Krzysztof, Ciechanowski Kazimierz
Clinical Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
Ann Transplant. 2010 Oct-Dec;15(4):15-20.
Immunological response following renal transplantation is a result of ischemia and reperfusion injury, which increase ICAM-1 and VCAM-1 endothelial expression. Reports suggest that there are genetic variations in ICAM-1 and VCAM-1 synthesis that can contribute to delayed graft function (DGF) and acute renal rejection after kidney transplantation. The aim of this study was to examine the effect of ICAM1 and VCAM1 gene polymorphisms on the early period after kidney transplantation.
MATERIAL/METHODS: The study enrolled 270 Caucasian renal transplant recipients (166 males, 104 females).
Genotyping of the rs5498 ICAM1 and the rs1041163 and rs3170794 VCAM1 gene polymorphisms was performed using real-time PCR.
The distribution of genotypes and alleles of the studied polymorphisms in patients with DGF and without DGF showed no statistically significant differences. The risk of acute rejection was significantly higher in patients with the rs5498 ICAM1 GG genotype than in carriers of the AG and AA genotypes (OR 3.01; 95% CI 1.51-6.00, p=0.003).
These results suggest that ICAM1 and VCAM1 gene polymorphisms play a minor role in pathogenesis of DGF. The rs5498 ICAM1 gene polymorphism is associated with increased risk of acute rejection of kidney allografts.
肾移植后的免疫反应是缺血再灌注损伤的结果，缺血再灌注损伤会增加细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）在内皮细胞上的表达。报告表明，ICAM-1和VCAM-1合成存在基因变异，这可能导致肾移植后移植肾功能延迟恢复（DGF）和急性肾排斥反应。本研究的目的是检测ICAM1和VCAM1基因多态性对肾移植早期的影响。
材料/方法：本研究纳入了270名白种人肾移植受者（166名男性，104名女性）。
采用实时聚合酶链反应（PCR）对ICAM1基因rs5498位点以及VCAM1基因rs1041163和rs3170794位点进行基因分型。
DGF患者和无DGF患者中所研究多态性的基因型和等位基因分布无统计学显著差异。rs5498 ICAM1基因GG基因型患者发生急性排斥反应的风险显著高于AG和AA基因型携带者（比值比3.01；95%可信区间1.51 - 6.00，p = 0.003）。
这些结果表明，ICAM1和VCAM1基因多态性在DGF发病机制中作用较小。rs5498 ICAM1基因多态性与同种异体肾移植急性排斥反应风险增加相关。
