Department of Ecology and Evolutionary Biology, University of California Irvine, Irvine, California, USA.
PLoS One. 2010 Sep 22;5(9):e12863. doi: 10.1371/journal.pone.0012863.
The hermaphroditic Mangrove Killifish, Kryptolebias marmoratus, is the world's only vertebrate that routinely self-fertilizes. As such, highly inbred and presumably isogenic "clonal" lineages of this androdioecious species have long been maintained in several laboratories and used in a wide variety of experiments that require genetically uniform vertebrate specimens. Here we conduct a genetic inventory of essentially all laboratory stocks of the Mangrove Killifish held worldwide. At 32 microsatellite loci, these stocks proved to show extensive interline differentiation as well as some intraline variation, much of which can be attributed to post-origin de novo mutations and/or to the segregation of polymorphisms from wild progenitors. Our genetic findings also document that many of the surveyed laboratory strains are not what they have been labeled, apparently due to the rather frequent mishandling or unintended mixing of various laboratory stocks over the years. Our genetic inventory should help to clarify much of this confusion about the clonal identities and genetic relationships of laboratory lines, and thereby help to rejuvenate interest in K. marmoratus as a reliable vertebrate model for experimental research that requires or can capitalize upon "clonal" replicate specimens.
雌雄同体的红树林咸水鱼，Kryptolebias marmoratus，是世界上唯一一种常规进行自体受精的脊椎动物。因此，这种雌雄同体的高度近亲繁殖和推测为同系的“克隆”品系在多个实验室中被长期维持，并被广泛用于需要遗传上均匀的脊椎动物标本的各种实验中。在这里，我们对全球范围内所有实验室饲养的红树林咸水鱼种群进行了遗传清查。在 32 个微卫星基因座上，这些种群表现出广泛的种系间分化以及一些种内变异，其中大部分可以归因于起源后的新突变和/或来自野生祖先的多态性的分离。我们的遗传发现还记录了许多被调查的实验室品系与其所标记的不符，这显然是由于多年来各种实验室种群的频繁处理不当或意外混合。我们的遗传清查应该有助于澄清关于实验室种群的克隆身份和遗传关系的许多混淆，从而有助于重新激发人们对 K. marmoratus 的兴趣，使其成为需要或可以利用“克隆”复制品的实验研究的可靠脊椎动物模型。
