Baseskioglu B, Akdogan B, Baydar D E, Ozen H
Hacettepe University Medical Faculty Urology Department, Ankara, Turkey.
Indian J Urol. 2010 Apr;26(2):206-12. doi: 10.4103/0970-1591.65390.
To analyze p53, Ki-67 and bcl-2 expressions immunohistochemically and their predictive role in biochemical recurrence after radical prostatectomy.
Seventy one patients who had undergone radical prostatectomy between 1992 and 2001 were randomly selected. Tissue microarrays were constructed from their radical prostatectomy specimens. They contained four cores from neoplastic and additional four cores from corresponding non-neoplastic regions. Gleason score ranged from 6-9, and pathological stage ranged from T2N0Mx to T3BN1. Staining for bcl-2 was scored visually taking percent negative, weak, moderate and strong positivity into consideration. Strong immunoreactivity was considered positive for p53. Ki-67 index was measured as the percentage of positive nuclei among tumor cells. Statistical analysis was performed to explore correlations between staining patterns and clinicopathological prognostic parameters.
The follow-up period extended from 13 to 112 months with a mean 60 (48 ± 23, 2) months. Of all, 38.02% had no evidence of disease, 52.1% were alive with disease and 9.8% were died during follow-up. The expression of p53, Ki-67 and bcl-2 in tumors were 39%, 76% and 5% respectively. While the secretory layer showed negative or weak bcl-2 staining in most cases, expression in basal cells was often stronger. Statistical analysis revealed differences in staining between normal and carcinoma for all three markers. There was no correlation between staining patterns and time to biochemical relapse. On the other hand, cases with higher Gleason sum showed the tendency for over expression of p53, Ki-67 and bcl-2 although the differences were not statistically different. Multivariate analysis revealed CMS group and seminal vesicle invasion as the independent predictors of PSA failure (log rank P = 0.0039 and P = 0.001, respectively).
The proteins bcl-2, p53 and Ki-67 were expressed at a different rate in normal and neoplastic prostate tissue. Bcl-2 was mainly expressed by basal cells in normal glands. p53 and Ki-67 expression were increased in most prostate carcinomas. However, overall expression levels did not correlate with biochemical recurrence in this study.
通过免疫组织化学分析p53、Ki-67和bcl-2的表达及其在前列腺癌根治术后生化复发中的预测作用。
随机选取1992年至2001年间接受前列腺癌根治术的71例患者。从其前列腺癌根治术标本构建组织芯片。芯片包含来自肿瘤组织的4个芯块以及相应非肿瘤区域的另外4个芯块。 Gleason评分范围为6 - 9分，病理分期范围为T2N0Mx至T3BN1。bcl-2染色通过视觉评分，考虑阴性、弱阳性、中等阳性和强阳性的百分比。p53强免疫反应性被视为阳性。Ki-67指数以肿瘤细胞中阳性细胞核的百分比来衡量。进行统计分析以探讨染色模式与临床病理预后参数之间的相关性。
随访期从13个月至112个月，平均60（48±23，2）个月。其中，38.02%无疾病证据，52.1%带瘤生存，9.8%在随访期间死亡。肿瘤中p53、Ki-67和bcl-2的表达分别为39%、76%和5%。虽然在大多数情况下分泌层bcl-2染色呈阴性或弱阳性，但基底细胞中的表达通常更强。统计分析显示所有三种标志物在正常组织和癌组织中的染色存在差异。染色模式与生化复发时间之间无相关性。另一方面，Gleason总分较高的病例显示p53、Ki-67和bcl-2有过表达的趋势，尽管差异无统计学意义。多因素分析显示CMS组和精囊侵犯是PSA失败的独立预测因素（对数秩检验P分别为0.0039和0.001）。
bcl-2、p53和Ki-67蛋白在正常和肿瘤性前列腺组织中的表达率不同。bcl-2主要由正常腺体中的基底细胞表达。大多数前列腺癌中p53和Ki-67表达增加。然而，在本研究中总体表达水平与生化复发无关。
