Cho Sung Hwan, Qiao Wen, Tsai Frank S, Yamashita Kenichi, Lo Yu-Hwa
Appl Phys Lett. 2010 Aug 30;97(9). doi: 10.1063/1.3481695. Epub 2010 Sep 2.
We describe a fluorescent detection technique for a lab-on-a-chip flow cytometer. Fluorescent emission is encoded into a time-dependent signal as a fluorescent cell or bead traverses a waveguide array with integrated spatial filters and color filters. Different from conventional colored filters with well-defined transmission spectral window, the integrated color filters are designed to have broad transmission characteristics, similar to the red-green-blue photoreceptors in the retina of human eye. This unique design allows us to detect multiple fluorescent colors with only three color filters based on the technique of color-space-time coding using only one single photomultiplier tube or avalanche photodetector.
我们描述了一种用于芯片实验室流式细胞仪的荧光检测技术。当荧光细胞或微珠穿过带有集成空间滤波器和滤色器的波导阵列时，荧光发射被编码为一个随时间变化的信号。与具有明确透射光谱窗口的传统滤色器不同，集成滤色器被设计成具有宽透射特性，类似于人眼视网膜中的红-绿-蓝光感受器。这种独特的设计使我们能够基于仅使用一个光电倍增管或雪崩光电探测器的颜色-空间-时间编码技术，仅用三个滤色器检测多种荧光颜色。
