Picot Julien, Guerin Coralie L, Le Van Kim Caroline, Boulanger Chantal M
Institut National de la Transfusion Sanguine, 75739, Paris Cedex 15, France,
Cytotechnology. 2012 Mar;64(2):109-30. doi: 10.1007/s10616-011-9415-0. Epub 2012 Jan 21.
Flow cytometry is a complete technology given to biologists to study cellular populations with high precision. This technology elegantly combines sample dimension, data acquisition speed, precision and measurement multiplicity. Beyond the statistical aspect, flow cytometry offers the possibility to physically separate sub-populations. These performances come from the common endeavor of physicists, biophysicists, biologists and computer engineers, who succeeded, by providing new concepts, to bring flow cytometry to current maturity. The aim of this paper is to present a complete retrospective of the technique and remind flow cytometry fundamentals before focusing on recent commercial instrumentation.
流式细胞术是一项提供给生物学家用于高精度研究细胞群体的完整技术。该技术巧妙地结合了样本维度、数据采集速度、精度和测量多样性。除了统计学方面，流式细胞术还提供了对亚群进行物理分离的可能性。这些性能源自物理学家、生物物理学家、生物学家和计算机工程师的共同努力，他们通过提供新的概念，成功地使流式细胞术发展到当前的成熟阶段。本文的目的是对该技术进行全面回顾，并在聚焦于近期商业仪器之前重温流式细胞术的基本原理。
