Biomicrofluidics. 2010 Sep 1;4(3):036502. doi: 10.1063/1.3481776.
Producing polymeric or hybrid microfluidic devices operating at high temperatures with reduced or no water evaporation is a challenge for many on-chip applications including polymerase chain reaction (PCR). We study sample evaporation in polymeric and hybrid devices, realized by glass microchannels for avoiding water diffusion toward the elastomer used for chip fabrication. The method dramatically reduces water evaporation in PCR devices that are found to exhibit optimal stability and effective operation under oscillating-flow. This approach maintains the flexibility, ease of fabrication, and low cost of disposable chips, and can be extended to other high-temperature microfluidic biochemical reactors.
在许多芯片应用中，包括聚合酶链反应（PCR），生产在高温下运行且减少或没有水蒸发的聚合或混合微流控设备是一个挑战。我们研究了通过玻璃微通道实现的聚合和混合设备中的样品蒸发，以避免水向用于芯片制造的弹性体扩散。该方法显著减少了 PCR 设备中的水分蒸发，这些设备在振荡流下表现出最佳的稳定性和有效运行。这种方法保持了一次性芯片的灵活性、易于制造和低成本，并且可以扩展到其他高温微流控生化反应器。
