Faculty of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, 514-0501, Japan.
Mar Biotechnol (NY). 2011 Jun;13(3):474-83. doi: 10.1007/s10126-010-9318-7. Epub 2010 Sep 29.
For pearl culture, nucleus and mantle grafts are implanted into the gonad of the host oyster. The epithelial cells of the implanted mantle graft elongate and surround the nucleus, and a pearl sac is formed. Shell matrix proteins secreted by the pearl sac play an important role in pearl formation. We studied the gene expression patterns of six shell matrix proteins (msi60, n16, nacrein, msi31, prismalin-14, and aspein) in the epithelial cells associated with pearl sac formation. There were differences in the expression patterns of the six genes in the epithelial cells, and the relative expression levels for msi60 and aspein differed between the mantle graft and pearl sac (48 days after implantation). Therefore, the gene expression patterns of the epithelial cells were genetically undetermined, and changed between before and after pearl sac formation. The gene expression patterns of the epithelial cells of the pearl sac may be regulated by the host oysters.
对于珍珠养殖，将核和套膜移植物植入宿主牡蛎的性腺中。植入的套膜移植物的上皮细胞伸长并包围核，形成珍珠囊。珍珠囊分泌的壳基质蛋白在珍珠形成中起重要作用。我们研究了与珍珠囊形成相关的上皮细胞中六种壳基质蛋白（msi60、n16、nacrein、msi31、prismalin-14 和 aspein）的基因表达模式。在这些上皮细胞中，六种基因的表达模式存在差异，msi60 和 aspein 的相对表达水平在套膜移植物和珍珠囊中存在差异（植入后 48 天）。因此，上皮细胞的基因表达模式是遗传上不确定的，在珍珠囊形成前后发生了变化。珍珠囊上皮细胞的基因表达模式可能受到宿主牡蛎的调节。
