Inoue Nariaki, Ishibashi Ryo, Ishikawa Takashi, Atsumi Takashi, Aoki Hideo, Komaru Akira
Faculty of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-0501, Japan.
Zoolog Sci. 2011 Jan;28(1):32-6. doi: 10.2108/zsj.28.32.
The production of a cultured pearl is the result of a complex interplay between the donor and recipient oysters. However, there is a paucity of information on the relationship between donor and recipient oyster gene expression patterns and pearl quality. Shell matrix proteins affect not only the formation of the shell, but also that of the pearls. We compared the gene expression patterns of five shell matrix proteins (msi60, nacrein, msi31, prismalin-14, and aspein) in the mantle edge (ME), which forms the prismatic layer, and the mantle center (MC), which forms the nacreous layer, between high- (HP) and low quality pearl- (LP) producing recipient oysters. After culturing for about two months, ME and MC tissues were collected from nine recipient oysters: four with HP, five with LP. In the ME, the average threshold cycle (ΔC(T)) for aspein was higher in HP than in LP (t-test, p = 0.03). Additionally, in the MC, the average ΔC(T) for msi60 was lower in HP than in LP (p = 0.06). This means the relative expression level of msi60 in the mantle of HP was higher than that of LP, and expression level of aspein in the mantle of HP was lower than that of LP. Pearl quality was closely related to the expression patterns of shell matrix protein genes of recipient oysters.
养殖珍珠的产生是供体和受体牡蛎之间复杂相互作用的结果。然而，关于供体和受体牡蛎基因表达模式与珍珠品质之间的关系，目前信息匮乏。贝壳基质蛋白不仅影响贝壳的形成，也影响珍珠的形成。我们比较了在形成棱柱层的外套膜边缘（ME）和形成珍珠层的外套膜中心（MC）中，高产珍珠（HP）和低产珍珠（LP）的受体牡蛎中五种贝壳基质蛋白（msi60、珍珠质、msi31、棱柱蛋白-14和天冬氨酸酶）的基因表达模式。养殖约两个月后，从九只受体牡蛎中收集ME和MC组织：四只HP牡蛎，五只LP牡蛎。在ME中，HP中aspein的平均阈值循环（ΔC(T)）高于LP（t检验，p = 0.03）。此外，在MC中，HP中msi60的平均ΔC(T)低于LP（p = 0.06）。这意味着HP外套膜中msi60的相对表达水平高于LP，HP外套膜中aspein的表达水平低于LP。珍珠品质与受体牡蛎贝壳基质蛋白基因的表达模式密切相关。
