Professional Graduate School of Oriental Medicine, Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Chonbuk 570-749, Republic of Korea.
Phytother Res. 2010 Oct;24(10):1489-95. doi: 10.1002/ptr.3131.
Vascular inflammation is involved in the initiation and progression of vascular diseases including atherosclerosis. While conducting in vitro screening of 600 medicinal plant extracts, an aqueous extract of Thuja orientalis (ATO) was found to exhibit antiinflammatory activity in human umbilical vein endothelial cells (HUVEC). In the current study, the antiinflammatory activity of ATO and possible mechanisms for this were investigated in HUVEC. Preincubation with ATO (20 μg/mL) suppressed tumor necrosis factor-α (TNF-α)-induced expression of adhesion molecules including intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-selectin at both the protein and mRNA levels. ATO also inhibited U937 monocyte adhesion to HUVEC stimulated by TNF-α. In addition, ATO attenuated TNF-α-induced p65 NF-κB translocation into the nucleus and phosphorylation of IκB-α. Furthermore, ATO significantly inhibited TNF-α-induced intracellular reactive oxygen species (ROS) production. Overall, the present data suggest that ATO can suppress TNF-α-induced vascular inflammatory processes, possibly via inhibition of ROS and NF-κB activation, in HUVEC.
血管炎症参与包括动脉粥样硬化在内的血管疾病的发生和发展。在对 600 种植物提取物进行体外筛选时，发现东方雪松（ATO）的水提取物在人脐静脉内皮细胞（HUVEC）中具有抗炎活性。在本研究中，研究了 ATO 的抗炎活性及其在 HUVEC 中的可能机制。ATO（20μg/mL）预孵育可抑制肿瘤坏死因子-α（TNF-α）诱导的黏附分子表达，包括细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）和 E-选择素，在蛋白质和 mRNA 水平上均有抑制作用。ATO 还可抑制 TNF-α刺激的 U937 单核细胞与 HUVEC 的黏附。此外，ATO 可减弱 TNF-α诱导的 p65 NF-κB 向核内易位和 IκB-α的磷酸化。此外，ATO 可显著抑制 TNF-α诱导的细胞内活性氧（ROS）的产生。总的来说，目前的数据表明，ATO 可通过抑制 ROS 和 NF-κB 激活来抑制 TNF-α诱导的血管炎症反应，从而抑制 TNF-α诱导的血管炎症过程，这可能在 HUVEC 中发生。
