Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Northern Ireland.
J Biomed Mater Res A. 2010 Dec 15;95(4):1142-9. doi: 10.1002/jbm.a.32931. Epub 2010 Sep 28.
The synthesis of nanophase hydroxyapatite (nHA) is of importance in the field of biomaterials and bone tissue engineering. The bioactive and osteoconductive properties of nHA are of much benefit to a wide range of biomedical applications such as producing bone tissue engineered constructs, coating medical implants, or as a carrier for plasmid DNA in gene delivery. This study aimed to develop a novel low-temperature dispersant-aided precipitation reaction to produce nHA particles (<100 nm), which are regarded as being preferable to micron-sized agglomerates of nHA. The variables investigated and optimized include the reaction pH, the rate of reactant mixing, use of sonication, order of addition, and concentration of the primary reactants, in addition, the effect of using poly(vinyl alcohol) (PVA) surfactant and Darvan 821A® dispersing agent during the reaction was also examined. It was found that by fine-tuning the synthesis parameters and incorporating the dispersing agent, monodisperse, phase-pure nano-sized particles under 100 nm were attained, suitable for clinical applications in bone regeneration.
纳米相羟基磷灰石（nHA）的合成在生物材料和骨组织工程领域具有重要意义。nHA 的生物活性和骨传导性对广泛的生物医学应用非常有益，例如生产组织工程化构建物、涂覆医疗植入物或作为质粒 DNA 在基因传递中的载体。本研究旨在开发一种新的低温分散剂辅助沉淀反应来生产纳米相羟基磷灰石颗粒（<100nm），这些颗粒被认为优于微米级的 nHA 团聚体。研究和优化的变量包括反应 pH 值、反应物混合速度、超声处理、添加顺序以及主要反应物的浓度，此外，还研究了在反应过程中使用聚乙烯醇（PVA）表面活性剂和 Darvan 821A®分散剂的效果。结果发现，通过精细调整合成参数并加入分散剂，可以获得单分散、相纯的纳米级颗粒，粒径小于 100nm，适用于骨再生的临床应用。
