Malarczyk E, Kochmańska-Rdest J
Department of Biochemistry, M. Curie-Skłodowska University, Lublin, Poland.
Acta Biochim Pol. 1990;37(1):145-8.
Vanillic acid at 0.2% concentration in the medium of Nocardia autotrophic DSM 43100 leads to cyclic production of guaiacol; protocatechuic and p-hydroxybenzoic acids as well as catechol appear at the same time in the medium instead of isovanillic acid, which accumulates at lower vanillic acid concentration. Transformation of catechol formed into guaiacol by methylation with formaldehyde, and successively into protocatechuic acid by carboxylation seems possible. Successive reactions of methylation/demethylation and carboxylation/decarboxylation result in cyclic production of guaiacol.
在嗜自养诺卡氏菌DSM 43100培养基中，0.2%浓度的香草酸会导致愈创木酚的循环产生；原儿茶酸、对羟基苯甲酸以及儿茶酚会同时出现在培养基中，而非异香草酸，异香草酸在较低香草酸浓度下会积累。儿茶酚通过与甲醛甲基化转化为愈创木酚，随后通过羧化转化为原儿茶酸似乎是可能的。甲基化/去甲基化和羧化/脱羧的连续反应导致愈创木酚的循环产生。
