香兰素的生物催化合成
Biocatalytic synthesis of vanillin.
作者信息
Li T, Rosazza J P
机构信息
Division of Medicinal and Natural Products Chemistry, and Center for Biocatalysis and Bioprocessing, College of Pharmacy, University of Iowa, Iowa City, Iowa 52242, USA.
出版信息
Appl Environ Microbiol. 2000 Feb;66(2):684-7. doi: 10.1128/AEM.66.2.684-687.2000.
Abstract
The conversions of vanillic acid and O-benzylvanillic acid to vanillin were examined by using whole cells and enzyme preparations of Nocardia sp. strain NRRL 5646. With growing cultures, vanillic acid was decarboxylated (69% yield) to guaiacol and reduced (11% yield) to vanillyl alcohol. In resting Nocardia cells in buffer, 4-O-benzylvanillic acid was converted to the corresponding alcohol product without decarboxylation. Purified Nocardia carboxylic acid reductase, an ATP and NADPH-dependent enzyme, quantitatively reduced vanillic acid to vanillin. Structures of metabolites were established by (1)H nuclear magnetic resonance and mass spectral analyses.
摘要
利用诺卡氏菌属菌株NRRL 5646的全细胞和酶制剂，研究了香草酸和邻苄基香草酸向香草醛的转化。在生长培养物中，香草酸脱羧生成愈创木酚（产率69%），并还原生成香草醇（产率11%）。在缓冲液中的静止诺卡氏菌细胞中，4-邻苄基香草酸转化为相应的醇产物，且未发生脱羧反应。纯化的诺卡氏菌羧酸还原酶是一种依赖ATP和NADPH的酶，可将香草酸定量还原为香草醛。通过核磁共振氢谱和质谱分析确定了代谢产物的结构。
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