Zhou San, Sekizaki Haruo, Yang Zhihong, Sawa Satoko, Pan Jun
Pharmacy Department, Medical Collage, Qingdao University, Qingdao 266021, China.
J Agric Food Chem. 2010 Oct 27;58(20):10972-8. doi: 10.1021/jf102694k. Epub 2010 Sep 29.
Hardseededness in annual wild soybean (Glycine soja Sieb. Et Zucc.) is a valuable trait that affects the germination, viability, and quality of stored seeds. Two G. soja ecotypes native to Shandong Province of China have been used to identify the phenolics in the seed coat that correlate with the seed hardness and seed germination. Three major phenolics from the seed coat were isolated and identified as epicatechin, cyanidin 3-O-glucoside, and delphinidin 3-O-glucoside. Of the three phenolics, only the change of epicatechin exhibited a significant positive correlation with the change of hard seed percentages both under different water conditions during seed development and under different gas conditions during seed storage. Epicatechin also reveals a hormesis-like effect on the seed germination of G. soja. Epicatechin is suggested to be functionally related to coat-imposed hardseededness in G. soja.
一年生野生大豆（Glycine soja Sieb. Et Zucc.）的硬实性是一种影响种子萌发、活力及贮藏种子质量的重要性状。来自中国山东省的两种野生大豆生态型被用于鉴定种皮中与种子硬度和种子萌发相关的酚类物质。从种皮中分离并鉴定出三种主要酚类物质，分别为表儿茶素、花青素3-O-葡萄糖苷和飞燕草素3-O-葡萄糖苷。在这三种酚类物质中，只有表儿茶素的变化在种子发育过程中的不同水分条件下以及种子贮藏过程中的不同气体条件下，均与硬实种子百分比的变化呈现出显著正相关。表儿茶素对野生大豆种子萌发还表现出类似 hormesis 的效应。表明表儿茶素在功能上与野生大豆种皮引起的硬实性相关。
