Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China; Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
Food Chem. 2017 May 15;223:104-113. doi: 10.1016/j.foodchem.2016.12.036. Epub 2016 Dec 18.
The effects of storage duration on the seed germination and metabolite profiling of soybean seeds with five different coloured coats were studied. Their germination, constituents and transcript expressions of isoflavones and free fatty acids (FFAs) were compared using chromatographic metabolomic profiling and transcriptome sequencing. The seed water content was characterized using nuclear magnetic resonance (NMR) relaxometry. Results showed that dark-coloured seeds were less inactivated than light-coloured seeds. The aglycone and β-glucoside concentrations of upstream constituents increased significantly, whereas the acetylglucosides and malonylglucosides of downstream constituents decreased with an increase in the storage period. FFAs increased considerably in the soybean seeds as a result of storage. These results indicate that dark-coloured soybean seeds have better storability than light-coloured seeds, and seed water content plays a role in seed inactivation. It was concluded that there are certain metabolic regularities that are associated with different coloured seed coats of soybeans under storage conditions.
研究了五种不同颜色种皮的大豆种子贮藏时间对种子萌发和代谢物谱的影响。采用色谱代谢组学分析和转录组测序比较了它们的萌发、成分以及异黄酮和游离脂肪酸（FFAs）的转录表达。利用核磁共振（NMR）弛豫度对种子含水量进行了表征。结果表明，深色种子比浅色种子的失活程度低。上游成分的糖苷元和β-葡萄糖苷浓度显著增加，而下游成分的乙酰葡萄糖苷和丙二酰葡萄糖苷随着贮藏时间的延长而减少。由于储存，大豆种子中的 FFAs 大量增加。这些结果表明，深色大豆种子比浅色种子具有更好的贮藏稳定性，种子含水量在种子失活中起作用。结论是，在储存条件下，不同颜色的大豆种皮存在一定的代谢规律。
