Wang Y, Wang A, Chen J, Zhao Y, Li X, Zang Y, Zhang G, Song Y
Department of Thoracic Surgery , No. 252 Hospital of P. L . A , Baoding 071000 , P. R. China.
Zhongguo Fei Ai Za Zhi. 1999 Mar 15;2(1):11-3. doi: 10.3779/j.issn.1009-3419.1999.01.04.
To investigate the clinical significance of MDR1 andMDRP gene expression in patients with non-small cell lung cancer (NSCLC) .
Using reverse transcription polymerase chain reaction (RT-PCR) technique , the expression of MDR1 and MDRP gene was determined in cancer and paracancerous tissues from 33 NSCLC patients.
Positive expression of MDR1 gene was 69.7% and 36.4% in lung cancer tissues and paracancerous tissues respectively , and a highly significant difference was observed between the two groups ( P < 0.01) . The expression levels of MDR1 mRNA were much higher than those in paracancerous tissues. The positivity rate of MDRP gene expression in cancer tissues was 63.6% , which was higher than 30.3% in paracancerous tissue. The gene expression levels in cancer tissues were also significantly higher than those in paracancerous tissues. The expression levels of MDR1 and MDRP in cancer tissues of the patients receiving pre-operative chemotherapy were remarkably higher than those of the patients not receiving chemotherapy ( P < 0.05) .
NSCLC had primary and acquired multidrug resistance. It was of important significance to detect MDR1 and MDRP gene expression in directing chemotherapy of lung carcinoma and in predicting prognosis.
探讨多药耐药基因1（MDR1）和多药耐药相关蛋白（MDRP）基因表达在非小细胞肺癌（NSCLC）患者中的临床意义。
采用逆转录聚合酶链反应（RT-PCR）技术，检测33例NSCLC患者癌组织及癌旁组织中MDR1和MDRP基因的表达。
MDR1基因在肺癌组织中的阳性表达率为69.7%，在癌旁组织中的阳性表达率为36.4%，两组间差异有高度显著性（P<0.01）。肺癌组织中MDR1 mRNA表达水平明显高于癌旁组织。MDRP基因在癌组织中的阳性表达率为63.6%，高于癌旁组织的30.3%，癌组织中基因表达水平也明显高于癌旁组织。术前接受化疗患者癌组织中MDR1和MDRP的表达水平明显高于未接受化疗患者（P<0.05）。
NSCLC存在原发性和获得性多药耐药。检测MDR1和MDRP基因表达对指导肺癌化疗及预测预后具有重要意义。
