Ruan Zheng, Hua Tianfang, Xue Yuehua
Department of Thoracic Surgery, The Affiliated No.1 Hospital, Jiaotong Univer- sity, Shanghai 200080, P.R.China.
Zhongguo Fei Ai Za Zhi. 2006 Dec 20;9(6):502-5. doi: 10.3779/j.issn.1009-3419.2006.06.05.
Fatty acid synthase (FAS) is differentially overexpressed and hyperactivated in many tumors and indicates poor prognosis. The aim of this study is to explore the expression of FAS mRNA and its significance in lung cancer tissues and paracancerous tissues, compared with normal lung tissues.
Fifty-five lung cancer tissues, 32 paracancerous tissues and 32 normal lung tissues were collected. Reverse transcription polymerase chain reaction (RT-PCR) and imaging analysis were applied to determine the FAS mRNA expression.
The positive rate of FAS mRNA expression was 72.7%, 62.5% and 25.0% in lung cancer tissues, paracancerous tissues and normal lung tissues, respectively (P < 0.01). The expression level of FAS mRNA was 0.885±0.06 in lung cancer tissues, 0.564±0.10 in paracancerous tissues and 0.259±0.04 in normal lung tissues. The expression level of FAS mRNA in lung cancer was significantly higher than that in paracancerous tissues and normal lung tissues (P < 0.01).
FAS mRNA is significantly overexpressed in lung cancer. The results suggest that overexpression of tumor lipid metabolism may play an important role in the oncogenesis and development of lung cancer.
脂肪酸合酶（FAS）在许多肿瘤中存在差异过表达和过度激活，提示预后不良。本研究旨在探讨FAS mRNA在肺癌组织、癌旁组织中的表达情况及其意义，并与正常肺组织进行比较。
收集55例肺癌组织、32例癌旁组织和32例正常肺组织。应用逆转录聚合酶链反应（RT-PCR）及图像分析检测FAS mRNA表达。
FAS mRNA在肺癌组织、癌旁组织和正常肺组织中的阳性表达率分别为72.7%、62.5%和25.0%（P<0.01）。肺癌组织中FAS mRNA表达水平为0.885±0.06，癌旁组织为0.564±0.10，正常肺组织为0.259±0.04。肺癌组织中FAS mRNA表达水平显著高于癌旁组织和正常肺组织（P<0.01）。
FAS mRNA在肺癌中显著过表达。结果提示肿瘤脂质代谢的过表达可能在肺癌的发生发展中起重要作用。
