Department of Biomedicine, University of Bergen, Bergen, Norway.
J Affect Disord. 2011 Mar;129(1-3):308-12. doi: 10.1016/j.jad.2010.08.028. Epub 2010 Sep 28.
Temperaments are stable personality traits that can be considered subsyndromal risk factors of psychiatric illnesses. The 5-HTTLPR polymorphism of the serotonin transporter gene has been found to be associated with affective temperaments, particularly the cyclothymic temperament, as measured with the Temperament Evaluation of Memphis, Pisa, Paris and San Diego-autoquestionnaire version (TEMPS-A). In this study we have attempted to replicate this finding in a population-based sample which is five times as large as the sample used in the original study.
The 21 items of the cyclothymic subscale of TEMPS-A was filled in by 691 individuals (404 females, 287 males, 18-40 years) randomly recruited from the general population. DNA was isolated from saliva, and the serotonin transporter polymorphism 5-HTTLPR was genotyped using the polymerase chain reaction and fragment analysis.
No significant association was found between 5-HTTLPR genotype and TEMPS-A score, neither when analysing by an additive allelic model nor when the different genotypes and allelic dominance were examined. Furthermore, no association was observed after gender stratification, or when TEMPS-A was analysed as a dichotomous measure, using a cut-off of ≥11 positive item responses.
Although being used in clinical settings, TEMPS-A has not been officially validated in Norway.
This study suggests that there is no association between the 5-HTTLPR polymorphism and cyclothymic temperament as measured by TEMPS-A.
气质是稳定的人格特质，可以被视为精神疾病的亚综合征风险因素。5-羟色胺转运体基因的 5-HTTLPR 多态性与情感气质有关，尤其是环性气质，这可以通过使用孟菲斯、比萨、巴黎和圣地亚哥自动问卷版（TEMPS-A）进行的气质评估来测量。在这项研究中，我们试图在一个基于人群的样本中复制这一发现，该样本是原始研究样本的五倍。
691 名（404 名女性，287 名男性，年龄 18-40 岁）随机从普通人群中招募的个体填写了 TEMPS-A 的环性亚量表的 21 个项目。从唾液中分离 DNA，并使用聚合酶链反应和片段分析对 5-羟色胺转运体多态性 5-HTTLPR 进行基因分型。
无论分析加性等位基因模型还是不同基因型和等位基因优势时，5-HTTLPR 基因型与 TEMPS-A 评分之间均无显著相关性。此外，在性别分层后，或当 TEMPS-A 作为二分类测量时，使用≥11 个阳性项目反应的截止值时，均未观察到关联。
尽管 TEMPS-A 已在临床环境中使用，但在挪威尚未正式验证。
本研究表明，5-HTTLPR 多态性与 TEMPS-A 测量的环性气质之间没有关联。
