脊髓损伤后大鼠和小鼠的神经病理学差异。
Neuropathological differences between rats and mice after spinal cord injury.
机构信息
Department of Neuroscience, Georgetown University Medical Center, Washington, DC, USA.
出版信息
J Magn Reson Imaging. 2010 Oct;32(4):836-46. doi: 10.1002/jmri.22323.
PURPOSE
To investigate the utility of noninvasive magnetic resonance imaging (MRI) protocols to demonstrate pathological differences between rats and mice after spinal cord injury (SCI). Rats and mice are commonly used to model SCI; however, histology and immunohistochemistry have shown differences in neuropathology between the two species, including cavity formation and scar/inflammatory responses.
MATERIALS AND METHODS
Moderate contusion SCI was performed on adult male rats and mice. At 28 days postinjury, animals underwent T1-weighted (T1W), with or without gadolinium contrast, or T2-weighted (T2W) magnetic resonance imaging (MRI), to be compared with histology at the same timepoint.
RESULTS
In both species, all MRI methods demonstrated changes in spinal cord anatomy. Immunohistochemistry indicated that T2W accurately reflected areas of inflammation and glial scar formation in rats and mice. Quantitation of lesion volume by histology and functional performance correlated best with T2W measurements in both species. Gadolinium contrast accurately reflected the blood-spinal cord-barrier permeability in both species, which appeared greater in rats than in mice.
CONCLUSION
These data demonstrate that MRI, with either a T1W or T2W protocol, can effectively distinguish pathological differences between rats and mice.
目的
研究非侵入性磁共振成像（MRI）方案在展示脊髓损伤（SCI）后大鼠和小鼠之间的病理差异方面的效用。大鼠和小鼠常用于 SCI 模型；然而，组织学和免疫组织化学已经显示出两种物种之间神经病理学的差异，包括空洞形成和疤痕/炎症反应。
材料和方法
对成年雄性大鼠和小鼠进行中度挫伤性 SCI。在损伤后 28 天，动物接受 T1 加权（T1W），有或没有钆对比，或 T2 加权（T2W）磁共振成像（MRI），与同一时间点的组织学进行比较。
结果
在两种物种中，所有 MRI 方法均显示脊髓解剖结构的变化。免疫组织化学表明，T2W 在大鼠和小鼠中准确反映了炎症和神经胶质疤痕形成的区域。通过组织学和功能表现对病变体积的定量与两种物种的 T2W 测量结果相关性最佳。钆对比准确反映了两种物种的血脊髓屏障通透性，大鼠中的通透性大于小鼠。
结论
这些数据表明，MRI 采用 T1W 或 T2W 方案，可有效区分大鼠和小鼠之间的病理差异。
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