大鼠脊髓损伤后神经病理变化的体内高分辨率磁共振成像
In vivo high-resolution MR imaging of neuropathologic changes in the injured rat spinal cord.
作者信息
Weber T, Vroemen M, Behr V, Neuberger T, Jakob P, Haase A, Schuierer G, Bogdahn U, Faber C, Weidner N
机构信息
Department of Physics, EP5, University of Würzburg, Würzburg, Germany.
出版信息
AJNR Am J Neuroradiol. 2006 Mar;27(3):598-604.
BACKGROUND AND PURPOSE
MR imaging is the most comprehensive noninvasive means to assess structural changes in injured central nervous system (CNS) tissue in humans over time. The few published in vivo MR imaging studies of spinal cord injury in rodent models by using field strengths < or = 7T suffer from low spatial resolution, flow, and motion artifacts. The aim of this study was to assess the capacity of a 17.6T imaging system to detect pathologic changes occurring in a rat spinal cord contusion injury model ex vivo and in vivo.
METHODS
Seven adult female Fischer 344 rats received contusion injuries at thoracic level T10, which caused severe and reproducible lesions of the injured spinal cord parenchyma. Two to 58 days postinjury, high-resolution MR imaging was performed ex vivo (2) or in vivo in anesthetized rats (5 spinal cord injured + one intact control animal) by using 2D multisection spin- and gradient-echo imaging sequences, respectively, combined with electrocardiogram triggering and respiratory gating.
RESULTS
The acquired images provided excellent resolution and gray/white matter differentiation without significant artifacts. Signal intensity changes, which were detected with ex vivo and in vivo MR imaging following spinal cord injury, could be correlated with histologically defined structural changes such as edema, fibroglial scar, and hemorrhage.
CONCLUSIONS
These results demonstrate that MR imaging at 17.6T allows high-resolution structural analysis of spinal cord pathology after injury.
背景与目的
磁共振成像（MR成像）是评估人类受伤中枢神经系统（CNS）组织随时间发生的结构变化的最全面的非侵入性手段。已发表的少数关于使用场强≤7T的啮齿动物模型脊髓损伤的体内MR成像研究存在空间分辨率低、血流和运动伪影的问题。本研究的目的是评估17.6T成像系统检测大鼠脊髓挫伤损伤模型离体和体内发生的病理变化的能力。
方法
7只成年雌性Fischer 344大鼠在胸段T10接受挫伤损伤，这导致了受损脊髓实质的严重且可重复的损伤。在损伤后2至58天，分别通过使用二维多层面自旋回波和梯度回波成像序列，并结合心电图触发和呼吸门控，对2只大鼠进行离体高分辨率MR成像，对5只脊髓损伤大鼠和1只完整对照动物进行体内高分辨率MR成像。
结果
所获取的图像提供了出色的分辨率和灰白质区分，且无明显伪影。脊髓损伤后通过离体和体内MR成像检测到的信号强度变化与组织学定义的结构变化如水肿、纤维胶质瘢痕和出血相关。
结论
这些结果表明，17.6T的MR成像能够对损伤后的脊髓病理进行高分辨率结构分析。
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