Department of Chemistry, Stanford University, Stanford, California 94305-5080, United States.
Org Lett. 2010 Nov 5;12(21):4820-3. doi: 10.1021/ol1019794.
To explore a new modular metal ion sensor design strategy, fluorophores and ligands were incorporated into short DNA-like oligomers. Compound 1 was found to function as a selective sensor for Ag(+) in aqueous buffer, where low micromolar concentrations of Ag(+) induce a red-shifted, turn-on fluorescence signal. Experiments with HeLa cells show that 1 can penetrate cells and yield a signal for intracellular Ag(+). This suggests a broadly applicable approach to developing sensors for a wide variety of cations.
为了探索一种新的模块化金属离子传感器设计策略,将荧光团和配体纳入短的类似 DNA 的寡聚物中。发现化合物 1 在水性缓冲液中可作为 Ag(+) 的选择性传感器,其中低微摩尔浓度的 Ag(+)诱导红移的开启荧光信号。用 HeLa 细胞进行的实验表明,1 可以穿透细胞并产生细胞内 Ag(+)的信号。这表明开发用于各种阳离子的传感器有一个广泛适用的方法。