Department of Chemistry and the Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.
J Am Chem Soc. 2009 Dec 23;131(50):18020-1. doi: 10.1021/ja906500m.
We present the synthesis and properties of Nickelsensor-1 (NS1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively responding to Ni(2+) in aqueous solution and in living cells. NS1 combines a BODIPY chromophore and a mixed N/O/S receptor to provide good selectivity for Ni(2+) over a range of biologically abundant metal ions in aqueous solution. In addition to these characteristics, confocal microscopy experiments further show that NS1 can be delivered into living cells and report changes in intracellular Ni(2+) levels in a respiratory cell model.
我们介绍了 Nickelsensor-1（NS1）的合成和性质，这是一种新的水溶性、开环荧光传感器，能够在水溶液中和活细胞中选择性地响应 Ni(2+)。NS1 将 BODIPY 生色团和混合的 N/O/S 受体结合在一起，在水溶液中对一系列生物丰富的金属离子具有良好的选择性。除了这些特性之外，共聚焦显微镜实验还进一步表明，NS1 可以递送到活细胞中，并在呼吸细胞模型中报告细胞内 Ni(2+)水平的变化。
