散发性肌萎缩侧索硬化症（sALS）患者外周血单个核细胞中的基因表达谱。
Gene expression profiling in peripheral blood mononuclear cells from patients with sporadic amyotrophic lateral sclerosis (sALS).
机构信息
University of California, San Francisco, San Francisco, CA 94110, USA.
出版信息
J Neuroimmunol. 2011 Jan;230(1-2):114-23. doi: 10.1016/j.jneuroim.2010.08.012. Epub 2010 Sep 29.
Abstract
The aim of this study was to identify gene expression profiles in peripheral blood mononuclear cells (PBMCs) from sporadic amyotrophic lateral sclerosis (sALS) patients to gain insights into the pathogenesis of ALS. We found that upregulation of LPS/TLR4-signaling associated genes was observed in the PMBCs from sALS patients after short-term cultivation, and that elevated levels of gene expression correlated with degree of peripheral blood monocyte activation and plasma LPS levels in sALS. Similar patterns of gene expression were reproduced in LPS stimulated PBMCs from healthy controls. These data suggest that chronic monocyte/macrophage activation may be through LPS/TLR4-signaling pathways in ALS.
摘要
本研究旨在鉴定散发性肌萎缩侧索硬化症（sALS）患者外周血单个核细胞（PBMCs）中的基因表达谱，以深入了解 ALS 的发病机制。我们发现，sALS 患者的 PBMCs 在短期培养后观察到 LPS/TLR4 信号相关基因的上调，并且基因表达水平的升高与 sALS 患者外周血单核细胞激活程度和血浆 LPS 水平相关。在健康对照的 LPS 刺激的 PBMCs 中也重现了类似的基因表达模式。这些数据表明，慢性单核细胞/巨噬细胞激活可能是通过 ALS 中的 LPS/TLR4 信号通路。
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