Reproductive Medicine Associates of New Jersey, Morristown, NJ, 07960, USA.
J Assist Reprod Genet. 2011 Jan;28(1):73-6. doi: 10.1007/s10815-010-9487-3. Epub 2010 Oct 1.
To report a birth of a healthy boy after long-term cryopreservation of oocytes by vitrification.
Clinical application.
IVF Center.
A 17 year-old female with secondary pulmonary hypertension caused by transposition of great vessels visited our center in 2002, and she wished oocytes cryopreservation to avoid possible sterility after the following category X medication treatment.
INTERVENTION(S): Vitrified oocytes on Electron Microscope (EM) grids were warmed after 5 years of storage. Surviving MII oocytes were microinjected for fertilization and two embryos were transferred into a gestational carrier day 5 after microinjection.
MAIN OUTCOME MEASURE(S): Survival, fertilization, cleavage, clinical pregnancy and delivery.
RESULT(S): Eleven out of fourteen oocytes (78.6%) survived warming. Eight Metaphase II (MII) oocytes and 3 in vitro matured oocytes were microinjected; all 11 oocytes (100%) fertilized and 2 embryos were transferred on day 5. A healthy baby boy weighing 3,600 g was delivered at 38 weeks of gestation. Live-birth rates per warmed oocyte and per injected oocyte were 7.1% and 9.1% respectively.
CONCLUSION(S): Cryopreservation after vitrification with EM grids maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth.
报道一例通过玻璃化冷冻长期保存卵母细胞后出生的健康男婴。
临床应用。
体外受精中心。
一名 17 岁女性，因大动脉转位继发肺动脉高压，于 2002 年就诊于本中心。她希望进行卵母细胞冷冻保存，以避免日后 X 类药物治疗可能导致的不孕。
将在电子显微镜（EM）载网上冷冻保存 5 年的卵母细胞进行解冻。存活的 MII 期卵母细胞进行显微注射受精，2 个胚胎于注射后第 5 天移植到妊娠载体中。
生存、受精、卵裂、临床妊娠和分娩。
14 个卵母细胞中有 11 个（78.6%）在解冻后存活。8 个 MII 期卵母细胞和 3 个体外成熟卵母细胞进行了显微注射；11 个卵母细胞（100%）均受精，2 个胚胎于第 5 天移植。妊娠 38 周时成功分娩出一名 3600g 的健康男婴。每个解冻卵母细胞和每个注射卵母细胞的活产率分别为 7.1%和 9.1%。
使用 EM 载网进行玻璃化冷冻保存可在长期储存后保持卵母细胞的发育能力，并成功获得活产。
