Ahn Geunseon, Park Jeong Hun, Kang Taeyun, Lee Jin Woo, Kang Hyun-Wook, Cho Dong-Woo
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea.
J Biomech Eng. 2010 Oct;132(10):104506. doi: 10.1115/1.4002429.
The aim of this study was to maximize oxygen diffusion within a three-dimensional scaffold in order to improve cell viability and proliferation. To evaluate the effect of pore architecture on oxygen diffusion, we designed a regular channel shape with uniform diameter, referred to as cylinder shaped, and a new channel shape with a channel diameter gradient, referred to as cone shaped. A numerical analysis predicted higher oxygen concentration in the cone-shaped channels than in the cylinder-shaped channels, throughout the scaffold. To confirm these numerical results, we examined cell proliferation and viability in 2D constructs and 3D scaffolds. Cell culture experiments revealed that cell proliferation and viability were superior in the constructs and scaffolds with cone-shaped channels.
本研究的目的是使三维支架内的氧气扩散最大化，以提高细胞活力和增殖能力。为了评估孔结构对氧气扩散的影响，我们设计了一种直径均匀的规则通道形状，称为圆柱形，以及一种具有通道直径梯度的新通道形状，称为圆锥形。数值分析预测，在整个支架中，圆锥形通道中的氧气浓度高于圆柱形通道。为了证实这些数值结果，我们检测了二维构建体和三维支架中的细胞增殖和活力。细胞培养实验表明，具有圆锥形通道的构建体和支架中的细胞增殖和活力更佳。
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