Sovová V, Bosch V, Cerná H
Folia Biol (Praha). 1978;24(3):199-205.
Lysates of various mammalian RSV-transformed cells labelled with 35S-methionine were tested for the presence of gs proteins and for the size of these molecules by means of antisera, using sodium dodecyl sulphate--polyacrylamide slab gel electrophoresis. In no case was the presence of gs proteins detected in the analyzed immunoprecipitates; only in RSH-mix cells a quantitative difference in the contrast of a 70 000-molecular weight protein was found. The autoradiograms of simultaneously tested immunoprecipitates of lysates of RSV-transformed chicken cells clearly showed the presence of p27 and after a short-term labelling also of the gs precursor molecule (pr76). The cause of the failure in detecting gs proteins by the used method in two mammalian RSV-transformed lines, in which the gs antigens were previously detected by complement fixation test, are discussed.
用35S-甲硫氨酸标记的各种哺乳动物劳氏肉瘤病毒（RSV）转化细胞的裂解物，通过抗血清，利用十二烷基硫酸钠-聚丙烯酰胺平板凝胶电泳，检测gs蛋白的存在情况以及这些分子的大小。在所分析的免疫沉淀物中，无一例检测到gs蛋白；仅在RSH混合细胞中，发现一种分子量为70000的蛋白质的条带强度存在定量差异。同时检测的RSV转化鸡细胞裂解物的免疫沉淀物的放射自显影片清楚地显示了p27的存在，短期标记后还显示了gs前体分子（pr76）的存在。讨论了在所使用的方法未能在两个哺乳动物RSV转化细胞系中检测到gs蛋白的原因，而之前通过补体结合试验在这两个细胞系中检测到了gs抗原。
