ICSI 对小鼠胚胎植入前基因表达和发育的影响。
Effect of ICSI on gene expression and development of mouse preimplantation embryos.
机构信息
Department of Obstetric, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, CA 94143, USA.
出版信息
Hum Reprod. 2010 Dec;25(12):3012-24. doi: 10.1093/humrep/deq266. Epub 2010 Oct 1.
BACKGROUND
In vitro culture (IVC) and IVF of preimplantation mouse embryos are associated with changes in gene expression. It is however not known whether ICSI has additional effects on the transcriptome of mouse blastocysts.
METHODS
We compared gene expression and development of mouse blastocysts produced by ICSI and cultured in Whitten's medium (ICSI(WM)) or KSOM medium with amino acids (ICSI(KSOMaa)) with control blastocysts flushed out of the uterus on post coital Day 3.5 (in vivo). In addition, we compared gene expression in embryos generated by IVF or ICSI using WM. Global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip.
RESULTS
Blastocysts from ICSI fertilization have a reduction in the number of trophoblastic and inner cell mass cells compared with embryos generated in vivo. Approximately 1000 genes are differentially expressed between ICSI blastocyst and in vivo blastocysts; proliferation, apoptosis and morphogenetic pathways are the most common pathways altered after IVC. Unexpectedly, expression of only 41 genes was significantly different between embryo cultured in suboptimal conditions (WM) or optimal conditions (KSOM(aa)).
CONCLUSIONS
Our results suggest that fertilization by ICSI may play a more important role in shaping the transcriptome of the developing mouse embryo than the culture media used.
背景
体外培养（IVC）和体外受精（IVF）的胚胎前植入与基因表达的变化有关。然而，尚不清楚 ICSI 是否对小鼠囊胚的转录组有额外的影响。
方法
我们比较了通过 ICSI 和在 Whitten 培养基（ICSI（WM））或含有氨基酸的 KSOM 培养基（ICSI（KSOMaa））中培养的囊胚的基因表达和发育，与在合子后第 3.5 天从子宫冲洗出的体内囊胚（in vivo）进行比较。此外，我们还比较了使用 WM 通过 IVF 或 ICSI 产生的胚胎的基因表达。使用 Affymetrix 430 2.0 芯片评估基因表达的整体模式。
结果
与体内产生的胚胎相比，ICSI 受精的囊胚的滋养层和内细胞团细胞数量减少。ICSI 囊胚和体内囊胚之间有大约 1000 个基因表达差异；增殖、凋亡和形态发生途径是 IVC 后最常改变的途径。出乎意料的是，在不理想条件（WM）或理想条件（KSOM（aa））下培养的胚胎之间，只有 41 个基因的表达有显著差异。
结论
我们的结果表明，ICSI 受精可能比使用的培养基更能影响发育中小鼠胚胎的转录组。
Zotero 插件