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用于分析埃及伊蚊发育过程中基因表达的整体原位杂交技术。

Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development.

作者信息

Haugen Morgan, Tomchaney Michael, Kast Kristopher, Flannery Ellen, Clemons Anthony, Jacowski Caitlin, Simanton Holland Wendy, Le Christy, Severson David, Duman-Scheel Molly

机构信息

Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USA.

出版信息

Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5509. doi: 10.1101/pdb.prot5509.

DOI:10.1101/pdb.prot5509
PMID:20889706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3076929/
Abstract

Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol for whole-mount in situ hybridization can be used to analyze gene expression in Ae. aegypti embryos and larvae, a critical aspect of understanding developmental gene function in this vector mosquito.

摘要

吸食血液的蚊子，包括登革热和黄热病的传播媒介埃及伊蚊，传播着世界上许多最致命的疾病。这些疾病在发展中国家再度出现，并对发达国家的疫情爆发构成明显威胁。最近的蚊子基因组计划激发了人们对通过基因操纵病媒昆虫来控制虫媒疾病可能性的兴趣，而调控发育的基因尤其受到关注。这种全胚胎原位杂交方案可用于分析埃及伊蚊胚胎和幼虫中的基因表达，这是了解这种病媒蚊子发育基因功能的关键方面。

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