Darquy S, Chicheportiche D, Capron F, Boitard C, Reach G
Diabetes Department, Hôtel-Dieu, Paris, France.
Horm Metab Res Suppl. 1990;25:209-13.
Microencapsulated rat islets of Langerhans (alginatepolylysine microcapsules) were implanted into the peritoneal cavity of diabetic mice (500 rat islets per mouse) in order 1) to evaluate the ability of this xenograft in correcting hyperglycemia in different models of diabetes and 2) to examine the implanted material recovered from the recipients after several weeks. 1) In the high-dose streptozotocin model in Balb/c mice (n = 14), 6 had a sustained (over one month) decrease in plasma glucose concentration from 401 +/- 7 to 171 +/- 7 mg/dl, with no effect in the other. 2) In the low dose streptozotocin model in C57BL/6J mice (n = 17), plasma glucose levels decreased in 9 mice, from 439 +/- 27 to 180 +/- 30 mg/dl, and remained below 250 mg/dl up to 60 days. In 8 mice, only a transient effect was observed. Empty capsule transplantation had no effect. Plasma insulin in successfully transplanted mice was higher in fed than in fasted state. 3) Microencapsulated rat islets had no effect on plasma glucose in male NOD mice made diabetic by cyclophosphamide treatment (n = 10). Thus, microencapsulated rat islets can improve the diabetic state in some, but not all diabetic mice. In this study, microcapsules were consistently surrounded by an inflammatory reaction, which remains a major concern.
将微囊化大鼠胰岛（海藻酸盐-聚赖氨酸微囊）植入糖尿病小鼠的腹腔（每只小鼠植入500个大鼠胰岛），目的如下：1）评估这种异种移植在不同糖尿病模型中纠正高血糖的能力；2）检查数周后从受体中回收的植入材料。1）在Balb/c小鼠的高剂量链脲佐菌素模型中（n = 14），6只小鼠的血糖浓度从401±7持续（超过1个月）降至171±7 mg/dl，其他小鼠则无效果。2）在C57BL/6J小鼠的低剂量链脲佐菌素模型中（n = 17），9只小鼠的血糖水平下降，从439±27降至180±30 mg/dl，并且在60天内一直低于250 mg/dl。在8只小鼠中，仅观察到短暂的效果。空胶囊移植无效果。成功移植的小鼠在进食状态下的血浆胰岛素水平高于禁食状态。3）微囊化大鼠胰岛对经环磷酰胺处理致糖尿病的雄性NOD小鼠（n = 10）的血糖没有影响。因此，微囊化大鼠胰岛可以改善部分但不是所有糖尿病小鼠的糖尿病状态。在本研究中，微囊始终被炎症反应包围，这仍然是一个主要问题。
