Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019, USA.
J Bacteriol. 2010 Dec;192(24):6494-6. doi: 10.1128/JB.01064-10. Epub 2010 Oct 1.
Modern methods to develop microbe-based biomass conversion processes require a system-level understanding of the microbes involved. Clostridium species have long been recognized as ideal candidates for processes involving biomass conversion and production of various biofuels and other industrial products. To expand the knowledge base for clostridial species relevant to current biofuel production efforts, we have sequenced the genomes of 20 species spanning multiple genera. The majority of species sequenced fall within the class III cellulosome-encoding Clostridium and the class V saccharolytic Thermoanaerobacteraceae. Species were chosen based on representation in the experimental literature as model organisms, ability to degrade cellulosic biomass either by free enzymes or by cellulosomes, ability to rapidly ferment hexose and pentose sugars to ethanol, and ability to ferment synthesis gas to ethanol. The sequenced strains significantly increase the number of noncommensal/nonpathogenic clostridial species and provide a key foundation for future studies of biomass conversion, cellulosome composition, and clostridial systems biology.
现代微生物基生物质转化过程的开发方法需要对所涉及的微生物有系统的了解。梭菌长期以来一直被认为是涉及生物质转化和生产各种生物燃料和其他工业产品的理想候选者。为了扩展与当前生物燃料生产相关的梭菌物种的知识库，我们对 20 个跨越多个属的物种进行了基因组测序。测序的大多数物种属于 III 类纤维素酶编码梭菌和 V 类产热厌氧杆菌科的糖化菌。选择这些物种是基于它们在实验文献中的代表性，作为模型生物，能够通过游离酶或纤维素酶降解纤维素生物质，能够快速发酵己糖和戊糖生成乙醇，以及能够发酵合成气生成乙醇。这些测序菌株大大增加了非共生/非致病性梭菌物种的数量，为未来的生物质转化、纤维素酶复合物组成和梭菌系统生物学研究提供了重要基础。
