一种从大肠杆菌中纯化RNA聚合酶全酶的快速方法。
A rapid method for the purification of RNA polymerase holoenzyme from Escherichia coli.
作者信息
Mehrpouyan M, Champney W S
机构信息
Department of Biochemistry, College of Medicine, East Tennessee State University, Johnson City 37614.
出版信息
J Biochem Biophys Methods. 1990 Nov-Dec;21(4):289-97. doi: 10.1016/0165-022x(90)90004-v.
PMID:2089071
Abstract
A method is described for the rapid purification of RNA polymerase holoenzyme from small amounts of Escherichia coli cells. Chromatography of a crude extract on a single-stranded DNA agarose column followed by gel filtration chromatography gave 95% pure holoenzyme. The enzyme had kinetic characteristics on T7 DNA identical to those of RNA polymerase purified by other more laborious procedures.
摘要
描述了一种从少量大肠杆菌细胞中快速纯化RNA聚合酶全酶的方法。粗提物在单链DNA琼脂糖柱上进行色谱分离，随后进行凝胶过滤色谱，得到了95%纯的全酶。该酶对T7 DNA的动力学特性与通过其他更繁琐方法纯化的RNA聚合酶相同。
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