Kumar K P, Chatterji D
Centre for Cellular and Molecular Biology, Hyderabad, India.
J Biochem Biophys Methods. 1988 Mar;15(5):235-40. doi: 10.1016/0165-022x(88)90010-3.
DNA-dependent RNA polymerase from Escherichia coli was purified further by elution through heparin-Sepharose CL-6B column after the enzyme was obtained, partially purified, using Burgess and Jendrisak's method [(1975)Biochemistry 14, 4634] The total yield of the pure protein was 10 mg from 50 g of E.coli cells. The method was found to be very reproducible and convenient. The enzyme preparation had 60% active molecules and the elongation rate of RNA synthesis by this enzyme was measured to be 11 bases/s over delta D111 T7 DNA.
通过Burgess和Jendrisak的方法[(1975)Biochemistry 14, 4634]获得并部分纯化大肠杆菌的依赖DNA的RNA聚合酶后，通过肝素-琼脂糖CL-6B柱洗脱进一步纯化。从50克大肠杆菌细胞中获得的纯蛋白总产量为10毫克。发现该方法具有很高的可重复性且方便实用。酶制剂中有60%的活性分子，经测定该酶在δD111 T7 DNA上进行RNA合成的延伸速率为11个碱基/秒。
