一种通过反相高效液相色谱法分离氨基酸苯硫代乙内酰脲的优化方法。
An optimized procedure for the separation of amino acid phenylthiohydantoins by reversed-phase HPLC.
作者信息
Persson B, Eaker D
机构信息
Department of Biochemistry, University of Uppsala, Sweden.
出版信息
J Biochem Biophys Methods. 1990 Nov-Dec;21(4):341-50. doi: 10.1016/0165-022x(90)90008-z.
PMID:2089073
Abstract
An optimization procedure for the separation of 24 PTH-amino acids by high-performance liquid chromatography on an inexpensive Merck Superspher Si 60 RP-8, (4.0 x 250 mm) column with PTH-Nle as an internal standard is described. The effects of pH, ionic strength, temperature and gradient were investigated. Using conventional HPLC equipment, the practical detection limit is about 5 pmol.
摘要
描述了一种在配备以PTH-Nle作为内标的廉价默克Superspher Si 60 RP-8（4.0×250 mm）柱上，通过高效液相色谱法分离24种PTH-氨基酸的优化程序。研究了pH值、离子强度、温度和梯度的影响。使用常规HPLC设备，实际检测限约为5皮摩尔。
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