Meuth J L, Fox J L
Anal Biochem. 1986 May 1;154(2):478-84. doi: 10.1016/0003-2697(86)90018-7.
Separation of the phenylthiohydantoin (PTH) derivatives of all 20 common amino acids is accomplished in approximately 11 min with excellent resolution by using high-pressure liquid chromatography. The chromatography is achieved at 50 degrees C on an Altex reversed-phase PTH-C18 column in an ammonium acetate-buffered acetonitrile, pH 4.5, mobile phase. Simple isocratic and linear gradient steps are used. Retention times for the various PTH-amino acids are very reproducible. Because the baseline is flat and free of background noise, PTH-amino acids can be detected in the low picomole range. The simplicity of this chromatographic system allows it to be easily automated.
使用高压液相色谱法，在约11分钟内即可实现对所有20种常见氨基酸的苯硫代乙内酰脲（PTH）衍生物的分离，分离度极佳。色谱分离在50℃下，于Altex反相PTH - C18柱上进行，流动相为pH 4.5的醋酸铵缓冲乙腈。采用简单的等度洗脱和线性梯度洗脱步骤。各种PTH - 氨基酸的保留时间具有很高的重现性。由于基线平坦且无背景噪声，可在低皮摩尔范围内检测到PTH - 氨基酸。该色谱系统操作简便，易于实现自动化。
