肌动蛋白丝加帽和切断蛋白gelsolin 与分子伴侣 CCT 之间的相互作用：非经典底物相互作用的证据。
Interactions between the actin filament capping and severing protein gelsolin and the molecular chaperone CCT: evidence for nonclassical substrate interactions.
机构信息
Department of Cell and Molecular Biology, Göteborgs Universitet, Medicinaregatan 9C, Gothenburg, Sweden.
出版信息
Cell Stress Chaperones. 2011 Mar;16(2):173-9. doi: 10.1007/s12192-010-0230-x. Epub 2010 Oct 2.
Abstract
CCT is a member of the chaperonin family of molecular chaperones and consists of eight distinct subunit species which occupy fixed positions within the chaperonin rings. The activity of CCT is closely linked to the integrity of the cytoskeleton as newly synthesized actin and tubulin monomers are dependent upon CCT to reach their native conformations. Furthermore, an additional role for CCT involving interactions with assembling/assembled microfilaments and microtubules is emerging. CCT is also known to interact with other proteins, only some of which will be genuine folding substrates. Here, we identify the actin filament remodeling protein gelsolin as a CCT-binding partner, and although it does not behave as a classical folding substrate, gelsolin binds to CCT with a degree of specificity. In cultured cells, the levels of CCT monomers affect levels of gelsolin, suggesting an additional link between CCT and the actin cytoskeleton that is mediated via the actin filament severing and capping protein gelsolin.
摘要
CCT 是分子伴侣 chaperonin 家族的一员，由 8 种不同的亚基组成，这些亚基在 chaperonin 环中占据固定位置。CCT 的活性与细胞骨架的完整性密切相关，因为新合成的肌动蛋白和微管蛋白单体依赖 CCT 来达到其天然构象。此外，CCT 涉及与组装/组装的微丝和微管相互作用的另一个作用正在出现。CCT 也已知与其他蛋白质相互作用，其中只有一些是真正的折叠底物。在这里，我们确定肌动蛋白丝重塑蛋白凝溶胶作为 CCT 的结合伙伴，尽管它不作为经典的折叠底物，但凝溶胶与 CCT 结合具有一定的特异性。在培养细胞中，CCT 单体的水平影响凝溶胶的水平，这表明 CCT 和肌动蛋白细胞骨架之间存在另一种联系，这种联系是通过肌动蛋白丝切断和加帽蛋白凝溶胶介导的。
相似文献
1
Interactions between the actin filament capping and severing protein gelsolin and the molecular chaperone CCT: evidence for nonclassical substrate interactions.肌动蛋白丝加帽和切断蛋白gelsolin 与分子伴侣 CCT 之间的相互作用：非经典底物相互作用的证据。
Cell Stress Chaperones. 2011 Mar;16(2):173-9. doi: 10.1007/s12192-010-0230-x. Epub 2010 Oct 2.
2
The molecular chaperone CCT modulates the activity of the actin filament severing and capping protein gelsolin in vitro.分子伴侣CCT在体外调节肌动蛋白丝切断和封端蛋白凝溶胶蛋白的活性。
Cell Stress Chaperones. 2016 Jan;21(1):55-62. doi: 10.1007/s12192-015-0637-5. Epub 2015 Sep 12.
3
The Molecular Chaperone CCT Sequesters Gelsolin and Protects it from Cleavage by Caspase-3.分子伴侣 CCT 将凝胶蛋白隔离并防止其被 caspase-3 切割。
J Mol Biol. 2022 Mar 15;434(5):167399. doi: 10.1016/j.jmb.2021.167399. Epub 2021 Dec 9.
4
The role of the molecular chaperone CCT in protein folding and mediation of cytoskeleton-associated processes: implications for cancer cell biology.分子伴侣 CCT 在蛋白质折叠和细胞骨架相关过程中的作用：对癌细胞生物学的影响。
Cell Stress Chaperones. 2019 Jan;24(1):17-27. doi: 10.1007/s12192-018-0949-3. Epub 2018 Dec 1.
5
Subunits of the chaperonin CCT interact with F-actin and influence cell shape and cytoskeletal assembly.伴侣蛋白 CCT 的亚基与 F-actin 相互作用，影响细胞形状和细胞骨架组装。
Exp Cell Res. 2010 Feb 15;316(4):543-53. doi: 10.1016/j.yexcr.2009.11.003. Epub 2009 Nov 12.
6
PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates.PhLP3通过与底物形成三元复合物来调节CCT介导的肌动蛋白和微管蛋白折叠。
J Biol Chem. 2006 Mar 17;281(11):7012-21. doi: 10.1074/jbc.M513235200. Epub 2006 Jan 16.
7
Interactions between monomeric CCTδ and p150: A novel function for CCTδ at the cell periphery distinct from the protein folding activity of the molecular chaperone CCT.单体 CCTδ 与 p150 之间的相互作用：CCTδ 在细胞外周的一种新功能，与分子伴侣 CCT 的蛋白质折叠活性不同。
Exp Cell Res. 2018 Sep 1;370(1):137-149. doi: 10.1016/j.yexcr.2018.06.018. Epub 2018 Jun 18.
8
A CapG gain-of-function mutant reveals critical structural and functional determinants for actin filament severing.一种CapG功能获得性突变体揭示了肌动蛋白丝切断的关键结构和功能决定因素。
EMBO J. 2006 Oct 4;25(19):4458-67. doi: 10.1038/sj.emboj.7601323. Epub 2006 Sep 14.
9
Distinct roles of four gelsolin-like domains of Caenorhabditis elegans gelsolin-like protein-1 in actin filament severing, barbed end capping, and phosphoinositide binding.秀丽隐杆线虫肌球蛋白样蛋白-1 的四个肌球蛋白样结构域在肌动蛋白丝的切断、突端封盖和磷酸肌醇结合中的不同作用。
Biochemistry. 2010 May 25;49(20):4349-60. doi: 10.1021/bi100215b.
10
The structure and evolution of eukaryotic chaperonin-containing TCP-1 and its mechanism that folds actin into a protein spring.真核细胞伴侣蛋白含有 TCP-1 的结构和进化及其将肌动蛋白折叠成蛋白弹簧的机制。
Biochem J. 2018 Oct 5;475(19):3009-3034. doi: 10.1042/BCJ20170378.
引用本文的文献
1
Loss of the E3 ubiquitin ligase TRIM67 alters the post-synaptic density proteome.E3泛素连接酶TRIM67的缺失会改变突触后致密蛋白质组。
MicroPubl Biol. 2024 Mar 1;2024. doi: 10.17912/micropub.biology.001118. eCollection 2024.
2
Loss of the E3 ubiquitin ligase TRIM67 alters the post-synaptic density proteome.E3泛素连接酶TRIM67的缺失会改变突触后致密蛋白质组。
bioRxiv. 2024 Jan 6:2024.01.05.574385. doi: 10.1101/2024.01.05.574385.
3
CCT6A knockdown suppresses osteosarcoma cell growth and Akt pathway activation in vitro.CCT6A 敲低抑制体外骨肉瘤细胞的生长和 Akt 通路的激活。
PLoS One. 2022 Dec 30;17(12):e0279851. doi: 10.1371/journal.pone.0279851. eCollection 2022.
4
The TRiCky Business of Protein Folding in Health and Disease.健康与疾病中蛋白质折叠的棘手问题
Front Cell Dev Biol. 2022 May 5;10:906530. doi: 10.3389/fcell.2022.906530. eCollection 2022.
5
Proteomic Changes Associated With Sperm Fertilizing Ability in Meat-Type Roosters.与肉用型公鸡精子受精能力相关的蛋白质组学变化
Front Cell Dev Biol. 2021 Apr 9;9:655866. doi: 10.3389/fcell.2021.655866. eCollection 2021.
6
Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.含伴侣蛋白TCP-1通过AKT-GSK3β-β-连环蛋白和XIAP-生存素通路促进癌症化疗耐药性和转移。
Cancers (Basel). 2020 Dec 21;12(12):3865. doi: 10.3390/cancers12123865.
7
Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells.CCT3的抑制作用会抑制乳腺癌细胞的增殖和迁移。
Cancer Cell Int. 2020 Jun 5;20:218. doi: 10.1186/s12935-020-01314-8. eCollection 2020.
8
The Molecular Chaperone CCT/TRiC: An Essential Component of Proteostasis and a Potential Modulator of Protein Aggregation.分子伴侣CCT/TRiC：蛋白质稳态的重要组成部分及蛋白质聚集的潜在调节因子
Front Genet. 2020 Mar 19;11:172. doi: 10.3389/fgene.2020.00172. eCollection 2020.
9
The role of the molecular chaperone CCT in protein folding and mediation of cytoskeleton-associated processes: implications for cancer cell biology.分子伴侣 CCT 在蛋白质折叠和细胞骨架相关过程中的作用：对癌细胞生物学的影响。
Cell Stress Chaperones. 2019 Jan;24(1):17-27. doi: 10.1007/s12192-018-0949-3. Epub 2018 Dec 1.
10
The molecular chaperone CCT modulates the activity of the actin filament severing and capping protein gelsolin in vitro.分子伴侣CCT在体外调节肌动蛋白丝切断和封端蛋白凝溶胶蛋白的活性。
Cell Stress Chaperones. 2016 Jan;21(1):55-62. doi: 10.1007/s12192-015-0637-5. Epub 2015 Sep 12.
本文引用的文献
1
Subunits of the chaperonin CCT interact with F-actin and influence cell shape and cytoskeletal assembly.伴侣蛋白 CCT 的亚基与 F-actin 相互作用，影响细胞形状和细胞骨架组装。
Exp Cell Res. 2010 Feb 15;316(4):543-53. doi: 10.1016/j.yexcr.2009.11.003. Epub 2009 Nov 12.
2
Defining the TRiC/CCT interactome links chaperonin function to stabilization of newly made proteins with complex topologies.定义TRiC/CCT相互作用组可将伴侣蛋白功能与具有复杂拓扑结构的新合成蛋白质的稳定性联系起来。
Nat Struct Mol Biol. 2008 Dec;15(12):1255-62. doi: 10.1038/nsmb.1515. Epub 2008 Nov 16.
3
Development of free-energy-based models for chaperonin containing TCP-1 mediated folding of actin.基于自由能模型的包含TCP-1的伴侣蛋白介导肌动蛋白折叠的研究进展
J R Soc Interface. 2008 Dec 6;5(29):1391-408. doi: 10.1098/rsif.2008.0185.
4
Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and cytoskeletal organisation.含TCP-1的伴侣蛋白（CCT）的活性：对细胞周期进程和细胞骨架组织的影响
Cell Stress Chaperones. 2009 Jan;14(1):23-31. doi: 10.1007/s12192-008-0057-x. Epub 2008 Jul 2.
5
The interaction network of the chaperonin CCT.伴侣蛋白CCT的相互作用网络。
EMBO J. 2008 Jul 9;27(13):1827-39. doi: 10.1038/emboj.2008.108. Epub 2008 May 29.
6
Downregulation of gelsolin family proteins counteracts cancer cell invasion in vitro.凝溶胶蛋白家族蛋白的下调在体外可对抗癌细胞侵袭。
Cancer Lett. 2007 Sep 18;255(1):57-70. doi: 10.1016/j.canlet.2007.03.023. Epub 2007 May 9.
7
Substantial CCT activity is required for cell cycle progression and cytoskeletal organization in mammalian cells.在哺乳动物细胞中，细胞周期进程和细胞骨架组织需要大量的CCT活性。
Exp Cell Res. 2006 Jul 15;312(12):2309-24. doi: 10.1016/j.yexcr.2006.03.028.
8
Quantitative actin folding reactions using yeast CCT purified via an internal tag in the CCT3/gamma subunit.使用通过CCT3/γ亚基中的内部标签纯化的酵母CCT进行的肌动蛋白定量折叠反应。
J Mol Biol. 2006 Jul 7;360(2):484-96. doi: 10.1016/j.jmb.2006.05.003. Epub 2006 May 17.
9
Actin interacts with CCT via discrete binding sites: a binding transition-release model for CCT-mediated actin folding.肌动蛋白通过离散的结合位点与CCT相互作用：一种用于CCT介导的肌动蛋白折叠的结合-转变-释放模型。
J Mol Biol. 2006 Jan 6;355(1):124-38. doi: 10.1016/j.jmb.2005.10.051. Epub 2005 Nov 9.
10
Gelsolin-induced epithelial cell invasion is dependent on Ras-Rac signaling.凝溶胶蛋白诱导的上皮细胞侵袭依赖于Ras-Rac信号传导。
EMBO J. 2002 Dec 16;21(24):6781-90. doi: 10.1093/emboj/cdf680.
Zotero 插件