Edelstein Arthur, Amodaj Nenad, Hoover Karl, Vale Ron, Stuurman Nico
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, USA.
Curr Protoc Mol Biol. 2010 Oct;Chapter 14:Unit14.20. doi: 10.1002/0471142727.mb1420s92.
With the advent of digital cameras and motorization of mechanical components, computer control of microscopes has become increasingly important. Software for microscope image acquisition should not only be easy to use, but also enable and encourage novel approaches. The open-source software package µManager aims to fulfill those goals. This unit provides step-by-step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.
随着数码相机的出现以及机械部件的自动化,显微镜的计算机控制变得越来越重要。用于显微镜图像采集的软件不仅应易于使用,还应支持并鼓励采用新颖的方法。开源软件包µManager旨在实现这些目标。本单元提供了逐步的协议,描述了如何开始使用µManager,以及该软件高级使用的一些起点。