在过氧化物酶和过氧化氢存在的情况下，5-羟色胺吲哚核的氧化及二聚体的形成。

Oxidation of the indole nucleus of 5-hydroxytryptamine and formation of dimers in the presence of peroxidase and H2O2.

作者信息

Heuther G, Reimer A, Schmidt F, Schuff-Werner P, Brudny M M

机构信息

Neurochemische Forschungsgruppe der Psychiatrischen Klinik, University of Göttingen, Federal Republic of Germany.

出版信息

J Neural Transm Suppl. 1990;32:249-57. doi: 10.1007/978-3-7091-9113-2_35.

DOI:10.1007/978-3-7091-9113-2_35

PMID:2089095

Abstract

5-Hydroxytryptamine (5-HT) is rapidly oxidized in the presence of peroxidase and H2O2. The major reaction product was isolated by gel chromatography and analyzed by mass spectroscopy. It is a 5-HT dimer formed under abstraction of two protons, most likely by the reaction at the C(4) position of two phenoxyradicals of 5-HT. In the presence of 5-HT an increased H2O2-consumption and a dose dependent reduction of the formation of reactive oxygen metabolites during H2O2 degradation was observed. The pattern of 5-HT reaction products separated by TLC was dependent on the H2O2 concentrations used. In the presence of albumen, plasma or tissue homogenates, massive binding of the 5-HT oxidation products to proteins was observed.

摘要

5-羟色胺（5-HT）在过氧化物酶和过氧化氢存在的情况下会迅速被氧化。主要反应产物通过凝胶色谱法分离，并通过质谱分析。它是一种在失去两个质子的情况下形成的5-HT二聚体，最有可能是由两个5-HT苯氧自由基在C(4)位置发生反应形成的。在5-HT存在的情况下，观察到过氧化氢消耗量增加，并且在过氧化氢降解过程中活性氧代谢产物的形成呈剂量依赖性减少。通过薄层层析分离的5-HT反应产物模式取决于所使用的过氧化氢浓度。在存在白蛋白、血浆或组织匀浆的情况下，观察到5-HT氧化产物与蛋白质大量结合。