识别可溶性和膜结合儿茶酚-O-甲基转移酶的单克隆抗体。

Monoclonal antibodies recognizing both soluble and membrane bound catechol-O-methyltransferase.

作者信息

Bertocci B, Garotta G, Zürcher G, Miggiano V, Da Prada M

机构信息

Pharmacological Department, University of Florence, Italy.

出版信息

J Neural Transm Suppl. 1990;32:369-74. doi: 10.1007/978-3-7091-9113-2_50.

DOI:10.1007/978-3-7091-9113-2_50

PMID:2089101

Abstract

Both cytosolic, soluble and membrane-bound catechol-O-methyl-transferase (COMT) from pig and rat liver or kidney were recognized by mouse monoclonal antibodies (MAbs) raised against soluble COMT isolated from pig liver. In ELISA, the MAbs Co 16 and Co 54 reacted better with the pig than with the rat enzyme. The MAb Co 60 showed good reactivity with both pig and rat COMT. In addition, all three MAbs recognize the soluble (23 kDa) as well as the membrane-bound (26 kDa) forms of the COMT enzyme.

摘要

来自猪和大鼠肝脏或肾脏的胞质可溶性和膜结合儿茶酚-O-甲基转移酶（COMT），都能被针对从猪肝中分离出的可溶性COMT产生的小鼠单克隆抗体（MAb）识别。在酶联免疫吸附测定（ELISA）中，单克隆抗体Co 16和Co 54与猪COMT的反应比对大鼠酶的反应更好。单克隆抗体Co 60对猪和大鼠的COMT均表现出良好的反应性。此外，所有这三种单克隆抗体都能识别COMT酶的可溶性形式（23 kDa）和膜结合形式（26 kDa）。