Department of Materials and Environmental Chemistry, Stockholm University, SE-106 91 Stockholm, Sweden.
Environ Toxicol Chem. 2010 Dec;29(12):2653-9. doi: 10.1002/etc.330. Epub 2010 Oct 1.
In the present study, muscle and liver tissue from 10 female Greenland sharks (Somniosus microcephalus) collected in Icelandic waters were analyzed for neutral and phenolic brominated organic compounds, including polybrominated diphenyl ethers (PBDEs) and the structurally related methoxylated (MeO) and hydroxylated (OH) PBDEs. Hydroxylated PBDEs exist both as natural products and as metabolites of the anthropogenic PBDEs, whereas MeO-PBDEs appear to exclusively be of natural origin. Other compounds examined were 2',6-dimethoxy-2,3',4,5'-tetrabromodiphenyl ether (2',6-diMeO-BDE68), 2,2'-dimethoxy-3,3',5,5'-tetrabromobiphenyl (2,2'-diMeO-BB80), 2,4,6-tribromoanisol (2,4,6-TBA) and 2,4,6-tribromophenol, all of natural origin, although 2,4,6-TBA and its phenolic counterpart may also be of anthropogenic origin. The major brominated organic compound was 6-MeO-BDE47, and ΣMeO-PBDE ranged from 49 to 210 ng/g fat in muscle and from 55 to 200 ng/g fat in liver tissue. Total concentrations of PBDEs were lower than ΣMeO-PBDE, in all but one sample, ranging between 7.3 to 190 and 9.9 to 200 ng/g fat in muscle and liver, respectively, and major congeners were BDE-47, BDE-99, and BDE-100. Polybrominated diphenyl ethers were analyzed using both high- and low-resolution mass spectrometry (MS) as a quality assurance, and the results from this comparison were acceptable. In accordance with previous work on Greenland sharks, no size/age-related accumulation was observed. Differences seen in concentrations were instead assumed to be a reflection of different feeding habits among the individuals. Phenolic compounds were only formed/retained in trace amounts in the Greenland shark. Among the phenolic compounds studied were 6-OH-BDE47, 2'-OH-BDE68, and 2,4,6-tribromophenol, all detected in liver and the latter two in muscle.
在本研究中，分析了在冰岛水域采集的 10 只雌性格陵兰鲨（Somniosus microcephalus）的肌肉和肝脏组织中中性和酚类溴化有机化合物，包括多溴二苯醚（PBDEs）和结构相关的甲氧基（MeO）和羟基（OH）PBDEs。羟基 PBDEs 既作为天然产物存在，也作为人为 PBDEs 的代谢物存在，而 MeO-PBDEs 似乎完全是天然产物。检查的其他化合物是 2'，6-二甲氧基-2,3'，4,5'-四溴二苯醚（2'，6-二 MeO-BDE68），2,2'-二甲氧基-3,3'，5,5'-四溴联苯（2,2'-二 MeO-BB80），2,4,6-三溴苯甲醚（2,4,6-TBA）和 2,4,6-三溴苯酚，全部为天然产物，尽管 2,4,6-TBA 和其酚类对应物也可能来自人为来源。主要的溴化有机化合物是 6-MeO-BDE47，ΣMeO-PBDE 范围分别为肌肉中的 49 至 210ng/g 脂肪和肝脏中的 55 至 200ng/g 脂肪。除了一个样本之外，所有样本中 PBDEs 的总浓度均低于 ΣMeO-PBDE，范围分别为肌肉和肝脏中的 7.3 至 190 和 9.9 至 200ng/g 脂肪，主要同系物为 BDE-47、BDE-99 和 BDE-100。使用高分辨率和低分辨率质谱（MS）对多溴二苯醚进行了分析，作为质量保证，并且比较结果是可以接受的。与以前对格陵兰鲨鱼的研究一致，没有观察到与大小/年龄相关的积累。相反，浓度的差异被认为是个体之间不同的饮食习惯的反映。酚类化合物仅以痕量形式在格陵兰鲨鱼中形成/保留。在所研究的酚类化合物中，有 6-OH-BDE47、2'-OH-BDE68 和 2,4,6-三溴苯酚，全部在肝脏中检测到，后两者在肌肉中检测到。
