采用酶联免疫吸附测定法测定毛花洋地黄毛状根培养物中的强心苷。

Determination of cardenolides in hairy root cultures of Digitalis lanata by enzyme-linked immunosorbent assay.

作者信息

Yoshimatsu K, Satake M, Shimomura K, Sawada J I, Terao T

机构信息

Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, Ibaraki, Japan.

出版信息

J Nat Prod. 1990 Nov-Dec;53(6):1498-502. doi: 10.1021/np50072a015.

DOI:10.1021/np50072a015

PMID:2089119

Abstract

A competitive ELISA technique using digoxin-specific antibody has been developed to determine digoxin and its related compounds in hairy root cultures of Digitalis lanata. The ELISA could detect 0.2-2 nM digoxin and closely related cardenolides. Hairy roots cultured in the dark accumulated very small amounts of cardenolides (0.02-0.07 micrograms/g dry wt), while the content of cardenolides in green hairy roots cultured in the light was increased maximally 600-fold (16.5 micrograms/g dry wt) compared to those in the dark.

摘要

已开发出一种使用地高辛特异性抗体的竞争性酶联免疫吸附测定（ELISA）技术，用于测定毛花洋地黄毛状根培养物中的地高辛及其相关化合物。该ELISA可检测出0.2 - 2 nM的地高辛及密切相关的强心甾类化合物。在黑暗中培养的毛状根积累的强心甾类化合物量非常少（0.02 - 0.07微克/克干重），而与黑暗中培养的相比，在光照下培养的绿色毛状根中强心甾类化合物的含量最多增加了600倍（16.5微克/克干重）。