固相萃取后用高效液相色谱法测定洋地黄叶中的强心甾类成分
High-performance liquid chromatographic determination of cardenolides in Digitalis leaves after solid-phase extraction.
作者信息
Wiegrebe H, Wichtl M
机构信息
Institut für Pharmazeutische Biologie, Philipps-Universität, Marburg/Lahn, Germany.
出版信息
J Chromatogr. 1993 Feb 5;630(1-2):402-7. doi: 10.1016/0021-9673(93)80478-q.
PMID:8445005
Abstract
For HPLC analysis of cardenolide glycosides of Digitalis lanata the separation of the main compounds from other constituents is useful. An improved method for doing this, based on solid-phase extraction on a C18 modified poly(styrene-divinylbenzene) polymer, is described. The presented assay permits quantitative estimation of more than 50 cardenolides in about 2 mg of dried leaf powder of Digitalis lanata with high speed and accuracy.
摘要
对于毛花洋地黄强心苷的高效液相色谱分析，将主要化合物与其他成分分离是很有用的。本文描述了一种基于在C18改性聚（苯乙烯 - 二乙烯基苯）聚合物上进行固相萃取的改进方法。所提出的分析方法能够以高速和高精度对约2毫克毛花洋地黄干叶粉中的50多种强心苷进行定量测定。
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