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白纹伊蚊C6/36细胞在披膜病毒科、黄病毒科、布尼亚病毒科和弹状病毒科虫媒病毒繁殖及分类中的应用

[The use of Aedes albopictus C6/36 cells in the propagation and classification of arbovirus of the Togaviridae, Flaviviridae, Bunyaviridae and Rhabdoviridae families].

作者信息

Figueiredo L T

机构信息

Departamento de Virologia do Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.

出版信息

Rev Soc Bras Med Trop. 1990 Jan-Mar;23(1):13-8. doi: 10.1590/s0037-86821990000100003.

Abstract

C6/36 Aedes albopictus cells were infected with Brazilian arbovirus from the families Togaviridae, Flaviviridae, Bunyaviridae and Rhabdoviridae. Replication was obtained with all the studied viruses and cytopathic effect was observed with some. Viral antigen was assayed in C6/36 cell cultures for antigen was assayed in C6/36 cells by an indirect immunofluorescence test using specific mouse immune ascitic fluid. Antigen production was detected in C6/36 cells infected with all the studied viruses. The author recommends the inoculation of C6/36 cell cultures for isolation of virus from the four studied families. The immunofluorescence technique is an important tool for classification and identification of virus growing in C6/36 cells.

摘要

用来自披膜病毒科、黄病毒科、布尼亚病毒科和弹状病毒科的巴西虫媒病毒感染白纹伊蚊C6/36细胞。所有研究的病毒都能在细胞中复制,部分病毒可观察到细胞病变效应。通过使用特异性小鼠免疫腹水,采用间接免疫荧光试验在C6/36细胞培养物中检测病毒抗原。在感染所有研究病毒的C6/36细胞中均检测到抗原产生。作者建议接种C6/36细胞培养物以从这四个研究的病毒科中分离病毒。免疫荧光技术是对在C6/36细胞中生长的病毒进行分类和鉴定的重要工具。

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