Muturi Ephantus J, Bara Jeffrey
Illinois Natural History Survey, Prairie Research Institute, University of Illinois at Urbana-Champaign, Champaign, Illinois, 61820, USA.
Parasit Vectors. 2015 Jan 30;8:65. doi: 10.1186/s13071-015-0667-y.
Mosquitoes transmit a number of arboviruses associated with disease outbreaks in humans and other animals. The majority of medically important arboviruses belong to three families: Togaviridae, Flaviviridae and Bunyaviridae. Several members of these families have overlapping distributions and share common vectors, increasing the potential for arboviral coinfections. This study examined how two model viruses: Sindbis virus (SINV, Togaviridae: Alphavirus) and dengue-4 virus (DENV-4, Flaviviridae: Flavivirus) may interact in C6/36 Aedes albopictus cells and in the mosquito vector Ae. albopictus.
C6/36 cells were coinfected, superinfected, or singly infected with SINV and DENV-4 and the two viruses quantified at different time points. Four to seven day old adult females of Ae. albopictus were also fed blood containing one or both viruses and viral infection and dissemination rates determined.
Sindbis virus suppressed replication of DENV-4 in C6/36 Ae. albopictus cells with greater inhibition occurring when the two arboviruses were inoculated simultaneously compared to sequentially. In addition, Ae. albopictus simultaneously exposed to both arboviruses had significantly lower DENV-4 infection and population dissemination rates compared to those exposed to DENV-4 alone.
These results suggest that certain Alphaviruses may interfere with DENV-4 transmission by suppressing its replication and increasing vector refractoriness. The findings provide important insights into the potential contribution of mixed arboviral infections to DENV transmission dynamics.
蚊子传播多种与人类和其他动物疾病暴发相关的虫媒病毒。大多数具有医学重要性的虫媒病毒属于三个科：披膜病毒科、黄病毒科和布尼亚病毒科。这些科的几个成员分布重叠且共享共同的传播媒介，增加了虫媒病毒合并感染的可能性。本研究考察了两种模型病毒：辛德毕斯病毒（SINV，披膜病毒科：甲病毒属）和登革热4型病毒（DENV-4，黄病毒科：黄病毒属）在白纹伊蚊C6/36细胞和蚊子媒介白纹伊蚊中的相互作用方式。
用SINV和DENV-4对C6/36细胞进行合并感染、重复感染或单独感染，并在不同时间点对这两种病毒进行定量。还让4至7日龄的白纹伊蚊成年雌性吸食含有一种或两种病毒的血液，并测定病毒感染率和传播率。
辛德毕斯病毒抑制了DENV-4在白纹伊蚊C6/36细胞中的复制，与顺序接种相比，两种虫媒病毒同时接种时抑制作用更强。此外，与单独接触DENV-4的白纹伊蚊相比，同时接触两种虫媒病毒的白纹伊蚊的DENV-4感染率和群体传播率显著更低。
这些结果表明，某些甲病毒可能通过抑制DENV-4的复制并增加媒介抗性来干扰其传播。这些发现为混合虫媒病毒感染对DENV传播动态的潜在影响提供了重要见解。
