Orsi A M, Vicentini C A, Dias S M, Michelin S C, Viotto M J
Departamento de Anatomia e de Morfologia, Universidade Estadual Paulista em Botucatu, SP, Brasil.
Rev Bras Biol. 1990 Feb;50(1):221-8.
The testicular stroma of the vampire bat including the testicular capsula and the lamina propria of the seminiferous tubuli, was strongly PAS-positive. This observation was a possible indication of great amounts of structural glycogen and other glycoconjugates at the level of smooth muscle cells; elongated contractile cells and/or collagen frameworks of the tunica albuginea and tubular lamina propria. In the last the basement membranes of the seminiferous tubules were particularly strongly PAS positive, as an indication of their neutral mucosubstances structural composition, previously described (Malmi et al., 1987). The epithelium lining from the cavitary and surface rete testis complex showed low reactivities to mucosubstances; total proteins and lipids and oxidative enzymes studied. Although the apical granulation at the rete testis epithelium showed an intense PAS reactivity with hypothesis of glycoprotein secretion, through the rete. The PAS, Sudan Black B, NADH, MDH and LDH reactions of the testicular interstitium seem correlate to steroid metabolism (biosynthesis and secretion), at the Leydig cells level. The seminiferous epithelium generally had low reactions to all the histochemical studies realized. Particularly in the adbasal compartment the histochemical localizations of NADH diaphorase and LDH were possible related to glycolytic activities and general carbohydrates metabolism, both enzymes, and hydrogen transport, the NADH. The strong PAS, diastase and PAS, and alcian blue pH 2.5 and PAS reactions observed in the adluminal seminiferous epithelium compartment were directly related to the spermatids acrosomal glycoconjugates structuration. Also the SDH localization at this level seems to be related to the mitochondrial activities at the middle piece level in the late spermatids.
吸血蝙蝠的睾丸基质，包括睾丸被膜和生精小管的固有层，PAS染色呈强阳性。这一观察结果可能表明，在平滑肌细胞水平存在大量的结构性糖原和其他糖结合物；白膜和生精小管固有层的细长收缩细胞和/或胶原纤维框架。最后，生精小管的基底膜PAS染色特别强阳性，这表明其具有中性黏液物质的结构组成，此前已有描述（马尔米等人，1987年）。来自睾丸网腔和表面复合体的衬里上皮对黏液物质、总蛋白、脂质和所研究的氧化酶反应较低。尽管睾丸网上皮的顶端颗粒显示出强烈的PAS反应性，推测可能是通过睾丸网分泌糖蛋白。睾丸间质的PAS、苏丹黑B、NADH、MDH和LDH反应似乎与间质细胞水平的类固醇代谢（生物合成和分泌）相关。生精上皮对所有已进行的组织化学研究反应普遍较低。特别是在基底隔室，NADH黄递酶和LDH的组织化学定位可能与糖酵解活性以及一般碳水化合物代谢、这两种酶以及氢转运（NADH）有关。在近腔生精上皮隔室观察到的强烈PAS、淀粉酶和PAS以及pH 2.5的阿尔辛蓝和PAS反应与精子细胞顶体糖结合物的结构直接相关。同样，该水平的SDH定位似乎与晚期精子细胞中段的线粒体活性有关。
