[Obtention of Trypanosoma cruzi clones of thin and thick epimastigotes and trypomastigotes living in the blood on various agar media].

Trypanosoma cruzi (EP strain) presents clonal growth when it is incorporated into at 0.7% agar and at 0.8% in surface. The colonies were obtained from a single parasite and in a relatively short time. The efficiency of plating was variable and depended on the type of agar medium. A maximal efficiency was obtained for at 0.7 agar/LIT-BHI and for at 0.8% agar/LIT-BHI-Blood. The sanguicolous tripomastigotes, either thin or thick, were able to generate colonies from single parasites, with 100% plating efficiency, higher than 80% corresponding to the epimastigotes coming from LIT medium.