Zaidenberg A, Tournier H, Schinella G, Buschiazzo H
Cátedra de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina.
Rev Latinoam Microbiol. 1995 Jan-Mar;37(1):71-7.
Morphogenesis of blood stream trypomastigotes in the cell free culture medium F69 at 37 degrees C for 10 days showed qualitative differences either with or without human plasma. Without human plasma, blood stream trypomastigotes performed only one cycle before disappearing and the culture kept growing as amastigotes and epimastigotes until the end of the experiment. In contrast, human plasma induced multiple cycles of transformation. The sequence was blood stream trypomastigotes, regressive parasites, amastigotes, progressive parasite and again trypomastigotes. Human plasma preserved the trypomastigote stage, produced a blockade of the epimastigote stage and inhibited the division of amastigotes. In this experimental model, human plasma modified the biological cycle of T. cruzi by inducing or inhibiting different stages.
在37摄氏度的无细胞培养基F69中培养10天，无论有无人类血浆，血流型锥鞭毛虫的形态发生都显示出质的差异。没有人类血浆时，血流型锥鞭毛虫在消失前仅进行一个周期，培养物持续以无鞭毛体和上鞭毛体的形式生长直至实验结束。相比之下，人类血浆诱导了多个转化周期。顺序为血流型锥鞭毛虫、退行性寄生虫、无鞭毛体、进行性寄生虫，然后又是锥鞭毛虫。人类血浆保留了锥鞭毛虫阶段，阻断了上鞭毛体阶段，并抑制了无鞭毛体的分裂。在这个实验模型中，人类血浆通过诱导或抑制不同阶段改变了克氏锥虫的生物周期。
