Momotani E, Yoshino T, Ishikawa Y, Nakajima Y
Hokkaido Branch Laboratory, National Institute of Animal Health, Sapporo, Japan.
Res Immunol. 1990 Oct;141(8):771-82. doi: 10.1016/0923-2494(90)90007-l.
Using immunohistochemistry, the differential distribution of the alpha subunit (S100 alpha) and beta subunit (S100 beta) of S100 protein was studied in mesenteric lymph nodes from normal or Mycobacterium paratuberculosis-infected cattle. In epithelioid cell granulomas, S100 alpha-positive epithelioid cells and some giant cells were scattered among S100 alpha-negative cells, which were predominant. The S100 beta-positive and -negative cells contained acid-fast bacilli. The presence of S100 beta-positive cells was not demonstrated in the granulomas. In normal component cells in the lymph nodes, follicular dendritic cells in the germinal centres and endothelium of lymphatic sinus and lymph vessels were positive for S100 alpha. S100 beta was positive only in the endothelial cells of blood vessels. Results shown in the present paper are discussed in light of results obtained in other work on human tissues using the same sources of antibodies.
利用免疫组织化学方法，研究了正常或副结核分枝杆菌感染牛肠系膜淋巴结中S100蛋白α亚基（S100α）和β亚基（S100β）的差异分布。在类上皮细胞肉芽肿中，S100α阳性的类上皮细胞和一些巨细胞散在于占主导地位的S100α阴性细胞之间。S100β阳性和阴性细胞均含有抗酸杆菌。在肉芽肿中未证实存在S100β阳性细胞。在淋巴结的正常组成细胞中，生发中心的滤泡树突状细胞以及淋巴窦和淋巴管的内皮细胞S100α呈阳性。S100β仅在血管内皮细胞中呈阳性。本文根据使用相同抗体来源对人体组织进行的其他研究结果对本研究结果进行了讨论。
