Sivakumar P, Tripathi B N, Singh N, Sharma A K
Division of Pathology, Indian Veterinary Research Institute, Izatnagar (Uttar Pradesh)-243 122 (India).
Vet Pathol. 2006 Jul;43(4):455-62. doi: 10.1354/vp.43-4-455.
Gross and histologic lesions of paratuberculosis were studied in water buffaloes. Small intestines and associated mesenteric lymph nodes of 405 water buffaloes were examined. Of these, 20 animals having visible changes of intestinal thickening, mucosal corrugations, and enlargement of mesenteric lymph nodes exhibited histologic alteration characteristics of mild to moderate granulomatous inflammation. The histologic lesions observed in these animals were classified into 3 grades on the basis of type of cellular infiltration, granuloma formation, and presence of acid-fast bacilli. Grade-1 lesions observed in 8 animals were marked by the presence of scattered epithelioid macrophages amid large number of lymphocytes in the intestinal villi and in the paracortical regions of the associated mesenteric lymph nodes. Another 8 animals classified under grade-2 revealed microgranulomas, infiltration with a larger number of epithelioid macrophages besides lymphocytes in the intestinal villi, and granulomas in the mesenteric lymph nodes. Grade-3 lesions observed in 4 animals were characterized by the presence of epithelioid granulomas and giant cells in the intestines and the mesenteric lymph nodes. The Ziehl-Neelsen's stained tissue sections revealed acid-fast bacilli in grade-3 and -2 animals and acid-fast granular debris in grade-1 animals. Among these 20 buffaloes, 14 (70%) were positive in the IS900 specific polymerase chain reaction and 6 (30%) were positive in the bacterial culture.
对水牛副结核病的大体和组织学病变进行了研究。检查了405头水牛的小肠及相关肠系膜淋巴结。其中，20头出现肠道增厚、黏膜皱襞和肠系膜淋巴结肿大等可见变化的动物表现出轻度至中度肉芽肿性炎症的组织学改变特征。根据细胞浸润类型、肉芽肿形成情况和抗酸杆菌的存在，将这些动物观察到的组织学病变分为3级。在8头动物中观察到的1级病变的特征是，在肠绒毛和相关肠系膜淋巴结的副皮质区大量淋巴细胞中存在散在的上皮样巨噬细胞。归入2级的另外8头动物显示有微肉芽肿，肠绒毛中除淋巴细胞外还有大量上皮样巨噬细胞浸润，肠系膜淋巴结中有肉芽肿。在4头动物中观察到的3级病变的特征是，在肠道和肠系膜淋巴结中存在上皮样肉芽肿和巨细胞。齐-尼氏染色的组织切片显示，3级和2级动物中有抗酸杆菌，1级动物中有抗酸颗粒碎片。在这20头水牛中，14头（70%）IS900特异性聚合酶链反应呈阳性，6头（30%）细菌培养呈阳性。
