6-脱氧-6-溴抗坏血酸抑制小鼠黑色素瘤细胞的生长。

6-Deoxy-6-bromo-ascorbic acid inhibits growth of mouse melanoma cells.

作者信息

Osmak M, Eckert-Maksić M, Pavelić K, Maksić Z B, Spavénti R, Beketić L, Kovacek I, Susković B

机构信息

Ruder Bosković Institute, Zagreb, Yugoslavia.

出版信息

Res Exp Med (Berl). 1990;190(6):443-9. doi: 10.1007/BF00000050.

DOI:10.1007/BF00000050

PMID:2089540

Abstract

The influence of 6-deoxy-6-bromo-ascorbic acid (6-Br-AA) and ascorbic acid (AA) on the growth of mouse melanoma cell line B16, mouse fibroblasts (L929), and human cervical carcinoma HeLa cells was examined by in vitro experiments. 6-Br-AA was added in concentrations 10(-1) to 10(-8)M and for incubation periods of 2, 18, 24 and 72 h. The present results indicate that 6-Br-AA exhibits a highly pronounced inhibiting effect on growth and DNA synthesis of melanoma cells. Inhibitory effect of 6-Br-AA was confirmed by the in vivo experiments. The tumor-suppressing effect on solid melanoma B16 was attained with 9 mg per mouse given three times daily for 16 days. Our preliminary results indicate that 6-Br-AA could serve as a potential antitumor agent.

摘要

通过体外实验研究了6-脱氧-6-溴抗坏血酸（6-Br-AA）和抗坏血酸（AA）对小鼠黑色素瘤细胞系B16、小鼠成纤维细胞（L929）和人宫颈癌HeLa细胞生长的影响。6-Br-AA的添加浓度为10（-1）至10（-8）M，孵育时间为2、18、24和72小时。目前的结果表明，6-Br-AA对黑色素瘤细胞的生长和DNA合成具有高度显著的抑制作用。体内实验证实了6-Br-AA的抑制作用。以每只小鼠9毫克的剂量，每天给药3次，持续16天，对实体黑色素瘤B16产生了肿瘤抑制作用。我们的初步结果表明，6-Br-AA可能作为一种潜在的抗肿瘤药物。