Economou E V, Kakabakos S E, Evangelatos G P, Ithakissios D S
National Center for Scientific Research Demokritos, Athens, Greece.
Steroids. 1990 Dec;55(12):545-50. doi: 10.1016/0039-128x(90)90050-l.
A radioimmunoassay method for the measurement of diethylstilbestrol directly on 50 microliters of human serum was established using a tritium-labeled radioligand and a double antibody as a separation reagent. The assay was sensitive (less than 0.17 micrograms/L), reproducible (intra-assay and interassay coefficient of variation values, 8.14% and 8.25%, respectively), and accurate (recovery up to 95%). Factors influencing the assay are identified and discussed.
